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首页> 外文会议>Sensing for agriculture and food quality and safety VI >Detection of pathogens in food using a SERS-based assay in just a few hours
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Detection of pathogens in food using a SERS-based assay in just a few hours

机译：在短短几个小时内使用基于SERS的检测方法检测食品中的病原体

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In 2011 Escherichia, Listeria, and Salmonella species infected over 1.2 million people in the United States, resulting in over 23,000 hospitalizations and 650 deaths, In January 2013 President Obama signed into law the Food and Drug Administration (FDA) Food Safety Modernization Act (FSMA), which requires constant microbial testing of food processing equipment and food to minimize contamination and distribution of food tainted with pathogens. The challenge to preventing distribution and consumption of contaminated foods lies in the fact that just a few bacterial cells can rapidly multiply to millions, reaching infectious doses within a few days. Unfortunately, current methods used to detect these few cells rely on similar growth steps to multiply the cells to the point of detection, which also takes a few days. Consequently, there is a critical need for an analyzer that can rapidly extract and detect foodborne pathogens at 1000 colony forming units per gram of food in 1-2 hours (not days), and with a specificity that differentiates from indigenous microflora, so that false alarms are eliminated. In an effort to meet this need, we have been developing an assay that extracts such pathogens from food, selectively binds these pathogens, and produces surface-enhanced Raman spectra (SERS) when read by a Raman analyzer. Here we present SERS measurements of these pathogens in actual food samples using this assay.
机译：2011年，美国的埃希氏菌，李斯特菌和沙门氏菌感染了超过120万人，导致23,000多例住院治疗和650例死亡，2013年1月，奥巴马总统签署了《食品药品管理局（FDA）食品安全现代化法案（FSMA）》 ），需要对食品加工设备和食品进行持续的微生物检测，以最大程度地减少污染和传播受病原体污染的食品。防止分配和食用受污染食品的挑战在于，只有少数细菌细胞可以迅速繁殖成百万，并在几天内达到传染剂量。不幸的是，用于检测这少数细胞的当前方法依赖于相似的生长步骤来使细胞繁殖到检测点，这也需要几天。因此，迫切需要一种能够在1-2小时（而不是几天）内以每克食物1000个菌落形成单位的速率快速提取和检测食源性病原体的分析仪，该分析仪应能与本地微生物区系区别开来，警报已消除。为了满足这一需求，我们一直在开发一种从食品中提取此类病原体，选择性结合这些病原体并通过拉曼分析仪读取时产生表面增强拉曼光谱（SERS）的测定法。在这里，我们介绍了使用此测定法对实际食品样品中这些病原体的SERS测量。

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