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Pulsed excitation system to measure the resonant frequency of magnetoelastic biosensors

机译:脉冲励磁系统测量磁弹性生物传感器的共振频率

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摘要

An electrical circuit was designed and tested to measure the resonant frequency of micron-scale magnetoelastic (ME) biosensors using a pulsed wave excitation technique. In this circuit, a square pulse current is applied to an excitation coil to excite the vibration of ME biosensors and a pick-up coil is used to sense the ME biosensor's mechanical vibration and convert it to an electrical output signal. The output signal is filtered and amplified by a custom designed circuit to allow the measurement of the resonant frequency of the ME biosensor from which the detection of specific pathogens can be made. As a proof-in-concept experiment, JRB7 phage-coated ME biosensors were used to detect different concentrations of Bacillus anthracis Sterne strain spores. A statistically significant difference was observed for concentrations of 5 × 10~2 spore/ml and above.
机译:设计并测试了电路,以使用脉冲波激发技术测量微米级磁弹性(ME)生物传感器的共振频率。在此电路中,方波电流施加到励磁线圈上,以激发ME生物传感器的振动,而拾波线圈则用于感测ME生物传感器的机械振动并将其转换为电输出信号。通过定制设计的电路对输出信号进行滤波和放大,以允许测量ME生物传感器的共振频率,从中可以检测出特定的病原体。作为概念验证实验,使用JRB7噬菌体包被的ME生物传感器检测不同浓度的炭疽芽孢杆菌斯特恩菌株孢子。对于5×10〜2孢子/ ml及更高的浓度,观察到统计学差异。

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