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High Throughput Pathogen Screening for Food Safety Using Magnetoelastic Biosensors

机译:磁弹性生物传感器用于食品安全的高通量病原体筛选

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In order to secure food safety, high throughput pathogen screening technique that can quickly identify and isolate unsafe contaminated foods has been long-desired. Recently, magnetoelastic (ME) free-standing biosensors have been investigated as a label-free wireless biosensor system for real-time pathogen detection. ME biosensor is composed of a ME resonator coated with a bio-molecular recognition element that binds specifically with a target pathogen. Once the biosensor comes into contact with the target pathogen, binding occurs, resulting in a decrease of the sensor's resonant frequency. Interrogated through magnetic signals, large amount of ME sensors can be deployed and monitored wirelessly. ME biosensors have been investigated to detect foodborne pathogens in cultures and liquid foods. Recently, it has been demonstrated that phage-based ME biosensors are able to directly detect Salmonella Typhimurium on food surfaces without the requirement of pre-analysis culture preparation. This paper will review the novel ME biosensor technique, including the detection principle, the characterization of the sensor performance, the deployment of multiple sensor detection and their applications, especially for food safety analysis. The ME biosensor technique has the potential to be a powerful pathogen screening tool for detecting contaminated food, identifying critical hazard points, and tracking contamination sources along the entire food supply chain.
机译:为了确保食品安全,一直期望能够快速识别和分离不安全的受污染食品的高通量病原体筛选技术。最近,磁弹性(ME)独立式生物传感器已被研究为用于实时病原体检测的无标签无线生物传感器系统。 ME生物传感器由涂有生物分子识别元件的ME共振器组成,该分子与目标病原体特异性结合。一旦生物传感器与目标病原体接触,就会发生结合,从而导致传感器共振频率降低。通过磁信号查询,可以无线部署和监视大量的ME传感器。已对ME生物传感器进行了研究,以检测培养物和液体食品中的食源性病原体。近来,已经证明基于噬菌体的ME生物传感器能够直接检测食物表面上的鼠伤寒沙门氏菌,而无需预先进行分析培养准备。本文将回顾新型的ME生物传感器技术,包括检测原理,传感器性能表征,多传感器检测的部署及其应用,尤其是在食品安全分析中。 ME生物传感器技术有可能成为一种强大的病原体筛选工具,用于检测受污染的食物,识别关键的危害点以及跟踪整个食物供应链中的污染源。

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