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首页> 外文会议>Sensing for agriculture and food quality and safety >Environmental effects on the production of Shiga-like toxins by Escherichia coli O157:H7 as revealed by sandwiched immuno-chemiluminescence detection
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Environmental effects on the production of Shiga-like toxins by Escherichia coli O157:H7 as revealed by sandwiched immuno-chemiluminescence detection

机译：夹心免疫化学发光检测表明，环境对大肠杆菌O157：H7产生志贺样毒素的环境影响

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摘要

We have developed a sandwiched immuno assay to detect sensitively Shiga-like toxins (SLTs) produced by Escherichia coli O157:H7. The method involved the capture of toxins by specific immuno magnetic beads followed by tagging the toxins with peroxidase-labeled anti E. coli O157:H7 antibody. Upon addition of proper substrate, peroxidase induced luminescence was used to measure the presence of SLTs. We have previously demonstrated that co-incubation of shiga toxin (SLT) producing E. coli O157:H7 with certain other bacteria can inhibit toxin production but does not affect the growth of the E. coli. We show here that media in which the cells have grown been centrifuged from (conditioned media) have similar effects on cell growth and SLT production. Adjusting the pH and adding nutrients to the conditioned media did not have any effect on the reduction of SLT produced. Bacteria communicate with each other via secreted sensing molecules. Several types of the molecules have been identified. However, the mechanisms of control remain to be established. This pattern for bacteria growth and toxin production is also observed when quorum-sensing molecules of homoserine lactone and indole are added to the media prior to inoculation.
机译：我们已经开发了一种夹心免疫测定法，以检测由大肠杆菌O157：H7产生的志贺样志贺样毒素（SLT）。该方法涉及通过特异性免疫磁珠捕获毒素，然后用过氧化物酶标记的抗大肠杆菌O157：H7抗体标记毒素。加入适当的底物后，过氧化物酶诱导的发光被用于测量SLT的存在。先前我们已经证明，产生志贺毒素（SLT）的大肠杆菌O157：H7与某些其他细菌的共培养可以抑制毒素的产生，但不影响大肠杆菌的生长。我们在这里显示，从中（条件培养基）离心细胞已生长的培养基对细胞生长和SLT产生具有相似的影响。调节pH值并向条件培养基中添加营养成分不会降低SLT的产生。细菌通过分泌的传感分子彼此通信。已经鉴定出几种类型的分子。但是，控制机制仍有待建立。当在接种前将高丝氨酸内酯和吲哚的群体感应分子添加到培养基中时，也观察到这种细菌生长和毒素产生的模式。

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