Polynucleotide primers and probes for the specific detection of E. coli0157:H7 and/or E. coli 0157:NM which produces verotoxin in samples areprovided. The primers and probes can be used in real time diagnostic assaysfor rapid detection of E. coli 0157:H7 and/or E. coli 0157:NM which producesverotoxin. The primers and probes can be used in real-time diagnostic assaysfor rapid detection of E. coli 0157:H7 and/or E. coli 0157:NM which producesverotoxin in a variety of situations and are capable of distinguishing E. coli0157:H7 and /or E. coli 0157:NM which produces verotoxin from other E. colistrains. Kits comprising the primers and probes are also provided.
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