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首页> 外文会议>Smart Medical and Biomedical Sensor Technology II; Progress in Biomedical Optics and Imaging; vol.5, no.32 >Bio-nano-optics for Cellular Investigations
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Bio-nano-optics for Cellular Investigations

机译：用于细胞研究的生物纳米光学

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We report results of our recent efforts to develop nano-tools to study proteins and their interactions in complex environments that exist on the cell membrane and inside the cells. Due to the spatial constraints imposed on the mobility of cell constituents, it is reasonable to expect that the nature and dynamics of the biomolecular interactions in a living cell would be substantially different from those routinely observed in dilute solutions. Nanotechnology has begun to provide tools with which to monitor processes that occur in membranes and intracellular regions. Nano-optics is a rich source of such emerging tools. Tapered optical fibers coated with metallic films can effectively confine excitation light to sub-wavelength linear dimensions and cubic nanometer excitation volumes. This leads not only to a resolution that exceeds the diffraction-limited values, but also to the elimination of the background signal. Thus, highly localized and specific regions of cellular function can be investigated. By immobilizing silver colloidal nanoparticles on such tapered fibers we have also fabricated surface enhanced Raman scattering (SERS) probes. Nanoprobes have been found to enable detection of fluorescent antibody molecules immobilized on a functionalized glass surface and polychromic quantum dots in picomolar solutions. In addition, we have successfully inserted nanoprobes with dimensions of 30-80 nm into both adherent insect and mammalian cells with maintenance of their viability. We summarize our development of optical nanoprobes with the motivation to detect cell-surface and intracellular proteins of the interleukin-5 system in native cellular environments, through quantum dot fluorescence and SERS.
机译：我们报告了我们最近努力开发纳米工具来研究蛋白质及其在细胞膜和细胞内部存在的复杂环境中的相互作用的结果。由于对细胞成分的移动性施加了空间限制，因此可以合理预期活细胞中生物分子相互作用的性质和动力学与稀溶液中常规观察到的显着不同。纳米技术已经开始提供工具来监测在膜和细胞内区域发生的过程。纳米光学是这类新兴工具的丰富来源。涂有金属膜的锥形光纤可以将激发光有效地限制在亚波长线性尺寸和立方纳米激发体积内。这不仅导致分辨率超过衍射极限值，而且导致消除背景信号。因此，可以研究细胞功能的高度局部和特定区域。通过将银胶体纳米颗粒固定在这种锥形纤维上，我们还制造了表面增强拉曼散射（SERS）探针。已经发现纳米探针能够检测固定在功能化玻璃表面上的荧光抗体分子和皮摩尔溶液中的多色量子点。此外，我们已经成功地将大小为30-80 nm的纳米探针插入粘附的昆虫和哺乳动物细胞中，并保持了它们的生存能力。我们总结了光学纳米探针的发展，其动机是通过量子点荧光和SERS检测天然细胞环境中白细胞介素5系统的细胞表面和细胞内蛋白。

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