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首页> 外文会议>Smart Medical and Biomedical Sensor Technology II; Progress in Biomedical Optics and Imaging; vol.5, no.32 >DNA-directed Assembly of Barcoded Nanowires onto Glass Slides for Biosensing Applications
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DNA-directed Assembly of Barcoded Nanowires onto Glass Slides for Biosensing Applications

机译：条码纳米线的DNA定向组装到用于生物传感应用的载玻片上

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摘要

With the increasing interest in simultaneous detection of specific DNA hybridization events, the development of methods to measure multiple DNA interactions at one time is of great importance. Conventional microarrays allow thousands of DNA hybridization interactions to be measured at once, however, this method of detection is limited by high cost as well as the stability and characteristic properties of fluorescent dyes. Here, barcoded nanowires are investigated as replacements for fluorophores on glass surfaces such as those used in microarrays. Potential advantages of nanowires include ease of reflectance-based optical read-out, the large number of tags available, and ability to distinguish multiple hybridizations occurring in a single DNA spot. A method of attaching DNA to glass microscope slides was developed which includes the use of a carboxy terminated silane to derivatize glass slides for DNA attachment. Also determined here is the efficiency of using nanowires as tags in complementary DNA hybridization events. An average of ～5% nonspecific binding was reported for nanowire attachment for all samples.
机译：随着对同时检测特定DNA杂交事件的兴趣日益浓厚，一次测量多种DNA相互作用的方法的开发非常重要。常规的微阵列允许一次测量数千个DNA杂交相互作用，但是，这种检测方法受到成本高昂以及荧光染料的稳定性和特性的限制。在这里，对条形码化的纳米线进行了研究，以替代玻璃表面（如微阵列中使用的表面）上的荧光团。纳米线的潜在优势包括基于反射率的光学读数的简便性，可用的大量标签以及区分单个DNA斑点中发生的多种杂交的能力。已经开发了将DNA附着于玻璃显微镜载玻片的方法，该方法包括使用羧基封端的硅烷衍生化玻璃载玻片以进行DNA附着。在这里还确定了在互补DNA杂交事件中使用纳米线作为标签的效率。据报道，所有样品的纳米线附着平均约5％的非特异性结合。

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