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首页> 外文会议>Society for Biomaterials Transaction of the 31st Annual Meeting vol.XXIX pt.2 >BIOPHYSICAL REGULATION OF STEM CELL DIFFERENTIATOR IS SURFACE DEPENDENT
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BIOPHYSICAL REGULATION OF STEM CELL DIFFERENTIATOR IS SURFACE DEPENDENT

机译：干细胞分化剂的生物物理调节取决于表面

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Osteogenesis in vivo occurs on a bone surface, however in vitro models to assess the mechanisms involved in the differentiation of mesenchymal stem cells (MSCs) into osteoblasts use conventional cell culture methods, including a tissue culture plastic substrate. We and others have shown the importance of substrate surface chemistry on osteoblast differentiation, but less is known about the requirements for MSC differentiation and commitment to an osteoblast phenotype. The purpose of this study was to compare MSC differentiation on tissue culture plastic to differentiation on a calcium phosphate ceramic substrate when cultured under osteogenic conditions. Typically, osteogenic assay conditions involve the use of a synthetic "osteogenic media" that contains dexamethasone and high levels of beta-glycerol phosphate. Addition of this media to confluent cultures of MSCs results in the formation of von Kossa positive nodules that express extracellular matrix proteins typical of bone. This is a time-dependent process that has been well characterized with respect to die inverse relationship between cell proliferation and differentiation, with the onset of an osteoblast phenotype at approximately 3 weeks post-confluence. The bone morphogen BMP-2, which induces osteogenesis in mesenchymal tissues in vivo, is used to stimulate osteoblastic differentiation in vitro. Pulsed electromagnetic fields (PEMF) are used clinically with the intent to stimulate MSCs, and studies using dernineralized bone matrix to induce osteogenesis in mouse muscle support the contention that PEMF enhances osteogenesis. Recent studies indicate that PEMF also affects MSCs in vitro. This study was based on the hypothesis that the osteoblastic differentiation of MSCs and the regulation of the process are substrate dependent. Accordingly, human MSCs were cultured on plastic or calcium phosphate disks and their response to osteogenic media determined in the presence and absence of a known activator of osteoblast differentiation, BMP-2, and a clinically used bone growth stimulator, PEMF.
机译：体内成骨发生在骨表面上，但是使用常规的细胞培养方法（包括组织培养塑料底物）来评估涉及间充质干细胞（MSC）分化为成骨细胞的机制的体外模型。我们和其他人已经显示了基质表面化学对成骨细胞分化的重要性，但对于MSC分化的要求和对成骨细胞表型的承诺知之甚少。这项研究的目的是比较在成骨条件下培养时，在组织培养塑料上的MSC分化与在磷酸钙陶瓷基质上的MSC分化。通常，成骨测定条件涉及使用包含地塞米松和高水平的β-甘油磷酸酯的合成“成骨培养基”。将该培养基添加到融合的MSCs培养物中会形成von Kossa阳性结节，该结节表达骨骼中典型的细胞外基质蛋白。这是一个随时间变化的过程，在细胞增殖与分化之间的逆向关系方面已经得到了很好的表征，在融合后大约3周开始出现成骨细胞表型。骨形态发生蛋白BMP-2在体内诱导间充质组织成骨，可用于刺激体外成骨细胞分化。临床上使用脉冲电磁场​​（PEMF）来刺激MSC，并且使用脱矿质骨基质诱导小鼠肌肉成骨的研究支持了PEMF增强成骨的观点。最近的研究表明，PEMF也会在体外影响MSC。这项研究基于以下假设，即MSC的成骨细胞分化和该过程的调节取决于底物。因此，将人MSC培养在塑料或磷酸钙盘上，并在存在和不存在已知的成骨细胞分化激活剂BMP-2和临床使用的骨生长刺激剂PEMF的情况下确定它们对成骨培养基的反应。

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