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Three-dimensional image contrast using biospeckle

机译:使用生物斑点的三维图像对比度

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The biospeckle laser (BSL) has been applied in many areas of knowledge and a variety of approaches has been presented to address the best results in biological and non-biological samples, in fast or slow activities, or else in defined flow of materials or in random activities. The methodologies accounted in the literature consider the apparatus used in the image assembling and the way the collected data is processed. The image processing steps presents in turn a variety of procedures with first or second order statistics analysis, and as well with different sizes of data collected. One way to access the biospeckle in defined flow, such as in capillary blood flow in alive animals, was the adoption of the image contrast technique which uses only one image from the illuminated sample. That approach presents some problems related to the resolution of the image, which is reduced during the image contrast processing. In order to help the visualization of the low resolution image formed by the contrast technique, this work presents the three-dimensional procedure as a reliable alternative to enhance the final image. The work based on a parallel processing, with the generation of a virtual map of amplitudes, and maintaining the quasi-online characteristic of the contrast technique. Therefore, it was possible to generate in the same display the observed material, the image contrast result and in addiction the three-dimensional image with adjustable options of rotation. The platform also offers to the user the possibility to access the 3D image offline.
机译:生物散斑激光(BSL)已应用于许多知识领域,并提出了多种方法来解决生物和非生物样品,快速或缓慢活动,或确定的物质流或生物样品中的最佳结果。随机活动。文献中介绍的方法考虑了图像组合中使用的设备以及收集到的数据的处理方式。图像处理步骤依次介绍了具有一阶或二阶统计分析以及收集到的不同大小数据的各种过程。一种以限定的流量(例如在活着的动物的毛细血管血流中)访问生物斑点的方法是采用图像对比技术,该技术仅使用被照明样品中的一张图像。该方法提出了一些与图像分辨率有关的问题,在图像对比度处理期间减少了这些问题。为了帮助可视化由对比度技术形成的低分辨率图像,这项工作提出了三维程序,作为增强最终图像的可靠选择。该工作基于并行处理,并生成虚拟的振幅图,并保持了对比技术的准在线特性。因此,可以在同一显示器中生成观察到的材料,图像对比度结果,并且可以生成具有可调旋转选项的三维图像。该平台还为用户提供了离线访问3D图像的可能性。

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