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首页> 外文会议>High-Speed Biomedical Imaging and Spectroscopy III: Toward Big Data Instrumentation and Management >High-speed particle tracking in microscopy using SPAD image sensors
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High-speed particle tracking in microscopy using SPAD image sensors

机译：使用SPAD图像传感器的显微镜中的高速粒子跟踪

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摘要

Single photon avalanche diodes (SPADs) are used in a wide range of applications, from fluorescence lifetime imaging microscopy (FLIM) to time-of-flight (ToF) 3D imaging. SPAD arrays are becoming increasingly established, combining the unique properties of SPADs with widefield camera configurations. Traditionally, the photosensitive area (fill factor) of SPAD arrays has been limited by the in-pixel digital electronics. However, recent designs have demonstrated that by replacing the complex digital pixel logic with simple binary pixels and external frame summation, the fill factor can be increased considerably. A significant advantage of such binary SPAD arrays is the high frame rates offered by the sensors (>100kFPS), which opens up new possibilities for capturing ultra-fast temporal dynamics in, for example, life science cellular imaging. In this work. we consider the use of novel binary SPAD arrays in high-speed particle tracking in microscopy. We demonstrate the tracking of fluorescent microspheres undergoing Brownian motion, and in intra-cellular vesicle dynamics, at high frame rates. We thereby show how binary SPAD arrays can offer an important advance in live cell imaging in such fields as intercellular communication, cell trafficking and cell signaling.
机译：单光子雪崩二极管（SPAD）的应用范围很广，从荧光寿命成像显微镜（FLIM）到飞行时间（ToF）3D成像。通过将SPAD的独特特性与宽视场摄像机配置相结合，SPAD阵列正变得越来越成熟。传统上，SPAD阵列的感光面积（填充系数）受到像素内数字电子设备的限制。但是，最近的设计表明，通过用简单的二进制像素和外部帧求和替换复杂的数字像素逻辑，可以显着提高填充因子。这种二进制SPAD阵列的一个显着优势是传感器提供的高帧速率（> 100kFPS），这为捕获例如生命科学细胞成像中的超快速时态动态开辟了新的可能性。在这项工作中。我们考虑在显微镜中使用新型二进制SPAD阵列进行高速粒子跟踪。我们展示了跟踪的荧光微球经历布朗运动，并在高帧频的细胞内囊泡动力学中。因此，我们展示了二进制SPAD阵列如何在诸如细胞间通信，细胞运输和细胞信号传导等领域的活细胞成像中提供重要的进展。

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  • 来源
  • 会议地点 San Francisco(US)
  • 作者

    Istvan Gyongy; Amy Davies; Allende Miguelez Crespo; Andrew Green; Neale A.W. Dutton; Rory R. Duncan; Colin Rickman; Robert K. Henderson; Paul A. Dalgarno;

  • 作者单位

    Institute for Integrated Micro and Nano Systems, School of Engineering, University of Edinburgh, Edinburgh EH9 3JL, UK;

    Institute of Biological Chemistry, Biophysics and Bioengineering, School of Engineering Physical Science, Heriot-Watt University, Edinburgh EH14 5AS, UK;

    Institute of Biological Chemistry, Biophysics and Bioengineering, School of Engineering Physical Science, Heriot-Watt University, Edinburgh EH14 5AS, UK;

    Institute of Biological Chemistry, Biophysics and Bioengineering, School of Engineering Physical Science, Heriot-Watt University, Edinburgh EH14 5AS, UK;

    Imaging Division, STMicroelectronics, Edinburgh EH3 5DA, UK;

    Institute of Biological Chemistry, Biophysics and Bioengineering, School of Engineering Physical Science, Heriot-Watt University, Edinburgh EH14 5AS, UK;

    Institute of Biological Chemistry, Biophysics and Bioengineering, School of Engineering Physical Science, Heriot-Watt University, Edinburgh EH14 5AS, UK;

    Institute for Integrated Micro and Nano Systems, School of Engineering, University of Edinburgh, Edinburgh EH9 3JL, UK;

    Institute of Biological Chemistry, Biophysics and Bioengineering, School of Engineering Physical Science, Heriot-Watt University, Edinburgh EH14 5AS, UK;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Particle tracking; Single-photon avalanche diode; Photon counting image sensor; Fluorescence microscopy;

    机译：粒子跟踪；单光子雪崩二极管；光子计数图像传感器；荧光显微镜;

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