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首页> 外文会议>Symposium on Biotechnology for Fuels and Chemicals; 20050501-04; Denver,CO(US) >Manipulating the Phenolic Acid Content and Digestibility of Italian Ryegrass (Lolium multiflorum) by Vacuolar-Targeted Expression of a Fungal Ferulic Acid Esterase
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Manipulating the Phenolic Acid Content and Digestibility of Italian Ryegrass (Lolium multiflorum) by Vacuolar-Targeted Expression of a Fungal Ferulic Acid Esterase

机译：通过以真菌为目标的真菌阿魏酸酯酶的表达来操纵意大利黑麦草（黑麦草）的酚酸含量和消化率

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摘要

In grass cell walls, ferulic acid esters linked to arabinosyl residues in arabinoxylans play a key role in crosslinking hemicellulose. Although such crosslinks have a number of important roles in the cell wall, they also hinder the rate and extent of cell wall degradation by ruminant microbes and by fungal glycohydrolyase enzymes. Ferulic acid esterase (FAE) can release both monomeric and dimeric ferulic acids from arabinoxylans making the cell wall more susceptible to further enzymatic attack. Transgenic plants of Lolium multiflorum expressing a ferulic acid esterase gene from Aspergillus niger, targeted to the vacuole under a constitutive rice actin promoter, have been produced following microprojectile bombardment of embryogenic cell cultures. The level of FAE activity was found to vary with leaf age and was highest in young leaves. FAE expression resulted in the release of monomeric and dimeric ferulic acids from cell walls on cell death and this was enhanced severalfold by the addition of exogenous β-1,4-endoxylanase. We also show that a number of plants expressing FAE had reduced levels of cell wall esterified monomeric and dimeric ferulates and increased in vitro dry-matter digestibility compared with nontransformed plants.
机译：在草细胞壁中，与阿拉伯木聚糖中阿拉伯糖基残基相连的阿魏酸酯在交联半纤维素中起关键作用。尽管此类交联在细胞壁中具有许多重要作用，但它们也阻碍了反刍微生物和真菌糖水解酶对细胞壁降解的速度和程度。阿魏酸酯酶（FAE）可以从阿拉伯木聚糖中释放单体和二聚阿魏酸，使细胞壁更容易受到进一步的酶攻击。在组成型水稻肌动蛋白启动子的作用下，针对黑液中表达了来自黑曲霉的阿魏酸酯酶基因的多花黑麦草的转基因植物，在对胚性细胞培养物进行了微粒轰击后产生。发现FAE活性水平随叶龄而变化，并且在幼叶中最高。 FAE表达导致细胞死亡时从细胞壁释放单体和二聚阿魏酸，并且通过添加外源β-1,4-内切木聚糖酶将其增强数倍。我们还显示，与未转化的植物相比，许多表达FAE的植物具有降低的细胞壁酯化单体和二聚体阿魏酸盐水平，并具有更高的体外干物质消化率。

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