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首页> 外文期刊>Applied biochemistry and biotechnology, Part A. enzyme engineering and biotechnology >Manipulating the Phenolic Acid Content and Digestibility of Italian Ryegrass (Lolium multiflorum) by Vacuolar-Targeted Expression of a Fungal Ferulic Acid Esterase
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Manipulating the Phenolic Acid Content and Digestibility of Italian Ryegrass (Lolium multiflorum) by Vacuolar-Targeted Expression of a Fungal Ferulic Acid Esterase

机译：通过以真菌为目标的真菌阿魏酸酯酶的表达操纵意大利黑麦草（黑麦草）的酚酸含量和消化率

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摘要

In grass cell walls,ferulic acid esters linked to arabinosyl residues in arabinoxylans play a key role in crosslinking hemicellulose.Although such crosslinks have a number of important roles in the cell wall,they also hinder the rate and extent of cell wall degradation by ruminant microbes and by fungal glycohydrolyase enzymes.Ferulic acid esterase (FAE) can release both monomeric and dimeric ferulic acids from arabinoxylans making the cell wall more susceptible to further enzymatic attack.Transgenic plants of Lolium multiflorum expressing a ferulic acid esterase gene from Aspergillus niger,targeted to the vacuole under a constitutive rice actin promoter,have been produced following microprojectile bombardment of embryogenic cell-cultures.The level of FAE activity was found to vary with leaf age and was highest in young leaves.FAE expression resulted in the release of monomeric-and dimeric ferulic acids from cell walls on cell death and this was enhanced severalfold by the addition of exogenous beta-1,4-endoxylanase.We also show that a number of plants expressing FAE had reduced levels of cell wall esterified monomeric and dimeric ferulates and increased in vitro dry-matter digestibility compared with nontransformed plants.
机译：在草细胞壁中，与阿拉伯木聚糖中阿拉伯糖基残基相连的阿魏酸酯在半纤维素的交联中起关键作用。尽管这种交联在细胞壁中具有许多重要作用，但它们也阻止了反刍动物对细胞壁降解的速度和程度。多叶黑麦草的转基因植物表达黑曲霉阿魏酸酯酶基因的转基因植物，主要​​针对于胚性细胞培养物的微粒轰击后产生了组成型水稻肌动蛋白启动子下的液泡。FAE活性水平随叶龄而变化，在幼叶中最高。FAE表达导致单体和来自细胞壁的二聚阿魏酸对细胞死亡的影响，并通过添加外源性β-1,4-内切木聚糖酶。我们还表明，与未转化的植物相比，许多表达FAE的植物的细胞壁酯化单体和二聚体阿魏酸水平降低，体外干物质消化率提高。

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