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首页> 外文会议>Symposium on Fermentation Biotechnology and 224th National Meeting of the American Chemical Society; Aug 18-22, 2002; Boston, Massachusetts >Vectors for Genetic Engineering of Corynebacteria
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Vectors for Genetic Engineering of Corynebacteria

机译：棒状杆菌基因工程的载体

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摘要

Corynebacterium glutamicum and related species are microorganisms of biotechnological significance. These bacteria have a long history of use for the production of fine chemicals such as amino acids and organic acids. Moreover, they have recently been contemplated for the production of xenogeneic proteins, such as proteins from Mycobacteria of medical importance, given the relatively close phylogenetic relationship that exists between these two genera. In this paper, we review various molecular tools available to manipulate Corynebacteria, provide DNA sequence information on several useful vectors, and report on novel shuttle vectors for the genetic engineering of these organisms. Particularly, this series of novel plasmids enables the use of chloramphenicol, kanamycin, gentamycin, or spectinomycin resistance for positive selection in Corynebacteria. These vectors complement the various molecular biology tools available to engineer these important bioconverters and thus pave the way to their increased industrial applications. Moreover, we present evidences corroborating the view that the B. stationis plasmid pbY503 belongs to a novel family of rolling-circle plasmids comprising pNG2 isolated from C. diphtheriae.
机译：谷氨酸棒杆菌和相关物种是具有生物技术意义的微生物。这些细菌用于生产精细化学物质（如氨基酸和有机酸）的历史悠久。此外，考虑到这两个属之间存在较近的系统发生关系，近来已考虑将它们用于生产异种蛋白，例如具有医学重要性的分枝杆菌的蛋白。在本文中，我们综述了可用于操作棒状杆菌的各种分子工具，提供了几种有用载体的DNA序列信息，并报道了用于这些生物体基因工程的新型穿梭载体。特别地，这一系列新质粒使得能够使用氯霉素，卡那霉素，庆大霉素或壮观霉素耐药性在棒状杆菌中进行阳性选择。这些载体补充了可用于工程化这些重要生物转化器的各种分子生物学工具，从而为它们的工业应用铺平了道路。此外，我们提供了证据，证实了站孢杆菌质粒pbY503属于新的滚环质粒家族，该家族包含从白喉衣原体分离的pNG2。

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