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首页> 外文会议>Symposium on Fermentation Biotechnology and 224th National Meeting of the American Chemical Society; Aug 18-22, 2002; Boston, Massachusetts >Submerged Fermentation of the Medicinal Mushroom Ganoderma lucidum for Production of Polysaccharide and Ganoderic Acid
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Submerged Fermentation of the Medicinal Mushroom Ganoderma lucidum for Production of Polysaccharide and Ganoderic Acid

机译：药用蘑菇灵芝的深层发酵生产多糖和灵芝酸

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摘要

Effects of oxygen supply and lactose feeding on submerged cultures of Ganoderma lucidum were investigated. A higher K_La value led to a higher biomass density and a higher productivity of both intracellular polysaccharide and ganoderic acid. In a stirred bioreactor, at an initial K_La of 78.2 h~(-1), a maximal cell concentration of 15.6 g/L by dry weight (DW) and a maximal intracellular polysaccharide (IPS) production of 2.2 g/L were obtained. An increase of initial AT_La led to a higher production and productivity of ganoderic acid (GA), and the GA production and productivity at an initial K_La value of 96.0 h~(-1) was 1.8-fold those at an initial K_La value of 16.4 h~(-1). In shake flasks, lactose feeding enhanced α-phosphoglucomutase activity, EPS production, phosphoglucose isomerase activity, and lactate accumulation. The information obtained may be useful for efficient large-scale production of these valuable bioactive products by the submerged cultures.
机译：研究了供氧和摄食乳糖对灵芝深层培养的影响。较高的K_La值导致较高的生物质密度和较高的细胞内多糖和灵芝酸生产率。在搅拌的生物反应器中，在初始K_La为78.2 h〜（-1）时，最大干细胞浓度（DW）为15.6 g / L，最大细胞内多糖（IPS）产量为2.2 g / L。初始AT_La的增加导致灵芝酸（GA）的产量和生产率更高，初始K_La值为96.0 h〜（-1）时的GA产量和生产率为初始K_La值为16.4时的1.8倍。 h〜（-1）。在摇瓶中，喂食乳糖可以增强α-磷酸葡萄糖变位酶活性，EPS的产生，磷酸葡萄糖异构酶的活性以及乳酸的积累。所获得的信息对于通过浸没培养物有效大规模生产这些有价值的生物活性产物可能有用。

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