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Cellular Toxicity of Different Classes of Adjuvants

机译:不同种类佐剂的细胞毒性

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The phytotoxicity of adjuvants depends on the amount of surfactant deposited per unit of leaf area, the penetration of the surfactant into the leaf, and the cellular toxicity. In this study we investigated the cellular toxicity by determining the leakage of electrolytes from potato discs. We included four adjuvant classes [alkylpolyglycosides (APG), polyoxyethylene fatty alcohols (FAL-EO), methylated polyoxyethylene fatty acids (FAC-EO-ME), and esterified polyoxyethylene glycerols (G-EO-E),]. In each class we included a lipophilic and a hydrophilic product by variation of the C-content of the alkyl chain (APG) or EO-content (other classes). Conductivity of the bathing media was measured at concentrations of 10~(-4),10~(-3),10~(-2),10~(-1), and 1% (w/v). A polyoxyethylene (10) nonylphenol was included as a reference compound. The cellular toxicity of the adjuvants started at concentrations >0.01% (FAL-EO and FAC-EO-ME) or >0.1% (APG). The glycerol derivatives (G-EO-E) were not toxic at all concentrations tested. The FAC-EO-ME class of adjuvants was the most toxic class; on average 65% of the total electrolyte content of the discs was released within 3h. The FAL-EO and the APG adjuvants were less toxic excepted the C_8-C~(10) APG giving a release of 56% at a concentration of 1%. In each adjuvant class the lipophilic product was less toxic than the more hydrophilic product. The results are discussed in relation to the physical-chemical properties of the adjuvants.
机译:佐剂的植物毒性取决于每单位叶面积沉积的表面活性剂的量,表面活性剂向叶片中的渗透以及细胞毒性。在这项研究中,我们通过确定马铃薯圆盘中电解质的泄漏来研究细胞毒性。我们包括四类佐剂[烷基多糖苷(APG),聚氧乙烯脂肪醇(FAL-EO),甲基化聚氧乙烯脂肪酸(FAC-EO-ME)和酯化聚氧乙烯甘油(G-EO-E)]。在每个类别中,我们通过改变烷基链的C含量(APG)或EO含量来包括亲脂性和亲水性产品(其他类别)。在10〜(-4),10〜(-3),10〜(-2),10〜(-1)和1%(w / v)的浓度下测量沐浴介质的电导率。包括聚氧乙烯(10)壬基苯酚作为参考化合物。佐剂的细胞毒性始于浓度> 0.01%(FAL-EO和FAC-EO-ME)或> 0.1%(APG)。甘油衍生物(G-EO-E)在所有测试浓度下均无毒。 FAC-EO-ME类佐剂毒性最高。平均而言,圆盘中总电解质含量的65%在3小时内释放。 FAL-EO和APG佐剂的毒性较小,但C_8-C〜(10)APG的浓度为1%时释放56%。在每种佐剂类别中,亲脂性产品的毒性均低于亲水性较高的产品。讨论了有关佐剂的物理化学性质的结果。

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