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首页> 外文会议>Symposium Proceedings vol.874; Symposium on Structure and Mechanical Behavior of Biological Materials; 20050329-31; San Francisco,CA(US) >The Influence of the Thermal Treatment of Hydroxylapatite Scaffolds on the Physical Properties and the Bone Cell Ingrowth Behaviour
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The Influence of the Thermal Treatment of Hydroxylapatite Scaffolds on the Physical Properties and the Bone Cell Ingrowth Behaviour

机译：羟基磷灰石支架热处理对物理性质和骨细胞向内生长的影响

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摘要

The material bone consists of a biopolymer matrix (collagen) reinforced with mineral nanoparticles (carbonated hydroxylapatite), forming a natural composite which builds up a dense shell on the exterior and a network of struts with a mean diameter of 200 μm in the core of many bones. The architecture of the foamy inner part of bones (spongiosa) is determined by loading conditions. The architecture strongly influences the mechanical properties of cellular solids together with the apparent density and the material it consists of. In addition, the ingrowth of bone cells into porous implants depends on pore size, size distribution and interconnectivity. From this it is clear that the possibility to design the architecture of a bone replacement material is beneficial from a biological as well as a mechanical point of view. Our approach uses rapid prototyping methods, ceramic gelcasting and sintering to produce cellular structures with designed architecture from hydroxylapatite and other bioceramics. The influence of sintering temperature and atmosphere on the physical properties of these scaffolds was investigated with x-ray diffraction and scanning electron microscopy. Furthermore, the cell ingrowth behaviour was determined in cell culture experiments, using the praeosteoblastic cell line MC3T3-E1, derived from mouse calvariae. The cell ingrowth behaviour was evaluated during a culture period of two and three weeks, by light microscopy and afterwards by histology after embedding and Giemsa-staining. The phase composition of the material was found to change with increasing sintering temperature and its surface characteristics was influenced by the sintering atmosphere. These changes also affected the cell ingrowth behaviour. In some experiments, the osteoblasts-like cells were found to cover the whole external and internal surface of the scaffold. The cells produced extracellular matrix consisting of collagen, which eventually filled nearly all the pores. In particular, the cells had the tendency to fill any crack or opening in the scaffolds, and to generally smooth the surfaces. In conclusion, rapid prototyping and ceramic gelcasting allows the freeform fabrication of porous bioceramics with controlled architecture. Such structures made of hydroxylapatit were found to support the growth of mouse osteoblasts.
机译：骨质材料由生物聚合物基质（胶原蛋白）和矿物纳米颗粒（碳酸羟基磷灰石）增强而成，形成一种天然复合材料，在外部形成致密的外壳，在许多核心中形成平均直径为200μm的支杆网络骨头。骨骼（海绵状）的泡沫内部的结构由加载条件决定。该结构极大地影响了多孔固体的机械性能以及表观密度及其组成的材料。另外，骨细胞向多孔植入物中的向内生长取决于孔径，尺寸分布和互连性。由此清楚的是，从生物学和机械的观点来看，设计骨替代材料的结构的可能性是有益的。我们的方法使用快速成型方法，陶瓷凝胶浇铸和烧结来生产具有羟基磷灰石和其他生物陶瓷设计结构的细胞结构。用X射线衍射和扫描电子显微镜研究了烧结温度和气氛对这些支架物理性能的影响。此外，在细胞培养实验中，使用源自小鼠颅盖的成骨细胞细胞系MC3T3-E1确定了细胞向内生长的行为。在两周和三周的培养期间，通过光学显微镜评价细胞的向内生长行为，然后在包埋和吉姆萨染色后通过组织学评价细胞向内生长。发现材料的相组成随烧结温度的升高而变化，并且其表面特性受烧结气氛的影响。这些变化也影响细胞向内生长的行为。在一些实验中，发现成骨细胞样细胞覆盖支架的整个外表面和内表面。细胞产生由胶原蛋白组成的细胞外基质，最终填充了几乎所有的孔。特别地，细胞倾向于填充支架中的任何裂缝或开口，并且通常使表面光滑。总之，快速原型制作和陶瓷凝胶浇铸可以自由控制结构可控的形式制造多孔生物陶瓷。发现由羟磷灰石制成的这种结构支持小鼠成骨细胞的生长。

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