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Bio-Hybrid Materials for Immunoassay-Based Sensing

机译:基于免疫分析的生物混合材料

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The process of encapsulating antibodies in sol-gel was used for sensing various hormones, specifically cortisol, insulin, and C-peptide. A sol-gel optical biosensor for cortisol has been developed for monitoring of crew health on-orbit during space missions. Our studies involving silica sol-gel materials with competitive immunoassays demonstrated linear calibration for cortisol in the range of 2-60 μg/dL, which covers the physiological range of cortisol blood concentration for an adult (2-28 μg/dL). The method of standard additions was used to analyze human serum samples sent to us from a NASA laboratory. Our sol-gel immunosensor values were typically within 20% of the values obtained using traditional immuno-binding techniques, with some values having less than a 5% error. Initial results are presented for sensing the hormones insulin and C-peptide.
机译:在溶胶-凝胶中包封抗体的过程用于感知各种激素,特别是皮质醇,胰岛素和C肽。已经开发出一种用于皮质醇的溶胶-凝胶光学生物传感器,用于在太空飞行任务中监视机组人员的在轨健康状况。我们对硅胶溶胶凝胶材料进行竞争性免疫分析的研究表明,皮质醇的线性校准范围为2-60μg/ dL,涵盖了成人皮质醇浓度的生理范围(2-28μg/ dL)。标准添加方法用于分析从NASA实验室发送给我们的人血清样品。我们的溶胶-凝胶免疫传感器值通常在使用传统免疫结合技术获得的值的20%以内,有些值的误差小于5%。初步结果显示了感测激素胰岛素和C肽。

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