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17 Study on antibacterial Activity of Hydrogel from Irradiated Silk Protein

机译:17辐射丝蛋白水凝胶的抗菌活性研究

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摘要

Hydrogels for biomedical application were prepared from solution blends of 3 percent silk protein and 3 percent, 10 percent poly(vinyl alcohol) (PVA) and followed with irradiation. Mixture of hydrogels were gamma irradiated at 10, 20, 30, 40 and 50 kGy under N_2 atmosphere. To clarify anti-bacterial activity of hydrogels, modified of the Agar disk diffusion method and American Association of Textile Chemists and Colorists, AATCC Test Method 90-1977, were carried out. The four kinds of bacteria such as Escherichia coli, Bacillus subtilis, Staphylococcus aureus and Staphylococcus epidermidis, were used. It was found that a 1:3 volume ratio of 3 percent silk protein and 3 percent PVA respectively, at 50 kGy irradiation, is suitable conditions for preparation hydrogels and trend to indicate the highest of an antibacterial activity against E. Coli, B. subtilis and S. aureus. However the antibacterial activity of hydrogels against S. epidermidis was not clearly. These results are very useful to expand the application of hydrogel from irradiated silk protein to the medical products.
机译:由3%的蚕丝蛋白和3%,10%的聚乙烯醇(PVA)的溶液混合物制备生物医学应用的水凝胶,然后进行辐照。在N_2气氛下以10、20、30、40和50kGyγ辐照水凝胶的混合物。为了阐明水凝胶的抗菌活性,对琼脂圆盘扩散法和美国纺织化学家和染色师协会的AATCC测试方法90-1977进行了修改。使用了四种细菌,例如大肠杆菌,枯草芽孢杆菌,金黄色葡萄球菌和表皮葡萄球菌。发现在50 kGy辐射下,分别为3%的丝蛋白和3%的PVA的1:3体积比是制备水凝胶的合适条件,并且趋势表明对大肠杆菌,枯草芽孢杆菌具有最高的抗菌活性。和金黄色葡萄球菌。然而,水凝胶对表皮葡萄球菌的抗菌活性尚不清楚。这些结果对于将水凝胶从辐照的丝蛋白扩展到医疗产品中非常有用。

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