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首页> 外文学位 >Cytocompatibility, antibacterial activity and biodegradability of self-assembling beta-hairpin peptide-based hydrogels for tissue regenerative applications.
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Cytocompatibility, antibacterial activity and biodegradability of self-assembling beta-hairpin peptide-based hydrogels for tissue regenerative applications.

机译：自组装的基于β-发夹肽的组织再生应用的细胞相容性，抗菌活性和生物降解性。

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摘要

Every year, millions of people suffer from tissue loss or failure. One approach to repair damaged or diseased tissue is through tissue/organ transplantation. However, one of the major problems which exist with this approach is that there are more people in need of a transplant than there are donors. Over the past several decades, scientists and doctors have come together to find a way to overcome this challenge. This collaboration has led to the development of biomimetic scaffolds, which closely mimic the desired tissue of interest to act as a substitute for the unfunctional tissue, with hopes to improve the quality of life.;The Schneider and Pochan labs have developed a biomimetic scaffold using self-assembling beta-hairpin peptides. The self-assembly event can be triggered in response to physiological conditions, which is dictated by the monomer, to form non covalently crosslinked mechanically rigid hydrogels. In vitro studies showed that hydrogels were cytocompatible and may not elicit a pro-inflammatory response from murine macrophages. These material properties show promise for the use of these hydrogels in tissue engineering.;When implanting a material into a host, a major concern is the introduction of infection. Infection, if not prevented or halted, results in poor tissue integration and function, ultimately leading to implant removal from the host. Interestingly, the beta-hairpin hydrogels were shown to exhibit antibacterial properties against pathogens commonly found in hospital environments. This inherently antibacterial hydrogel is advantageous because it may help decrease or diminish bacterial contamination when implanted in vivo, which may help to increase the success of implants. Also, a unique and exciting feature of these peptide-based hydrogels is their ability to shear-thin and self-heal. Hydrogels can be directly formed in a syringe and be subsequently delivered to a tissue defect in a minimally invasive manner where they will recover to their original mechanical rigidity. The resultant syringe-delivered gel was also shown to possess antibacterial properties. Aside from the material's inherent antibacterial activity, these peptide-based scaffolds display degradation that can be controlled using an exogenously added enzyme. This suggests that by using peptide design, the gel network degradation can be controlled to allow for the proper formation of functional tissue. The work described in this thesis shows these described attributes, as well as, the potential of these peptide-based gels for use as tissue substitutes.
机译：每年，成千上万人遭受组织损失或衰竭。修复受损或患病组织的一种方法是通过组织/器官移植。但是，这种方法存在的主要问题之一是需要移植的人多于捐助者。在过去的几十年中，科学家和医生一起找到了克服这一挑战的方法。这项合作促成了仿生支架的开发，该仿生支架紧密模仿所需的目标组织以替代功能不佳的组织，以期改善生活质量。; Schneider和Pochan实验室开发了一种仿生支架，使用自组装的β-发夹肽。可以响应于由单体决定的生理条件来触发自组装事件，以形成非共价交联的机械刚性水凝胶。体外研究表明，水凝胶具有细胞相容性，可能不会引起鼠巨噬细胞的促炎反应。这些材料特性显示了在组织工程中使用这些水凝胶的前景。当将材料植入宿主时，主要的关注是感染的引入。如果不加以预防或阻止，感染将导致不良的组织整合和功能，最终导致植入物从宿主体内移出。有趣的是，β-发夹水凝胶显示出对医院环境中常见病原体的抗菌性能。这种固有的抗菌水凝胶是有利的，因为当在体内植入时，它可以帮助减少或减少细菌污染，这可以帮助增加植入的成功率。而且，这些基于肽的水凝胶的独特和令人兴奋的功能是其剪切变薄和自我修复的能力。水凝胶可以直接在注射器中形成，然后以微创的方式输送到组织缺损处，在那里它们将恢复到其原始的机械刚度。还显示了所得的注射器递送的凝胶具有抗菌特性。除了材料固有的抗菌活性外，这些基于肽的支架还表现出降解作用，可以使用外源添加的酶进行控制。这表明通过使用肽设计，可以控制凝胶网络的降解以允许功能组织的适当形成。本论文中描述的工作显示了这些描述的属性，以及这些基于肽的凝胶用作组织替代物的潜力。

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  • 作者

    Salick, Daphne Ann.;

  • 作者单位

    University of Delaware.;

  • 授予单位 University of Delaware.;
  • 学科 Chemistry Organic.;Engineering Materials Science.
  • 学位 Ph.D.
  • 年度 2009
  • 页码 209 p.
  • 总页数 209
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 有机化学;工程材料学;
  • 关键词

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