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THE INACTIVATION OF PROBIOTIC BACTERIA DURING (SPRAY) DRYING

机译:喷雾干燥过程中对细菌的活化

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摘要

An experimental approach to assess the microbial viability within single particles is presented.It comprises a single droplet drying method and a subsequent novel live-dead screening methodology.The drying method involves the drying of a single droplet deposited on a flat,hydrophobic surface under well-defined drying conditions and carrier formulations.(Semi-)dried particles were subjected to rehydration,fluorescence staining,and live/dead enumeration using fluorescence microscopy.The novel screening methodology provided accurate survival percentages in line with conventional plating enumeration and was evaluated in single droplet drying experiments with Lactobacillus plantarum WCFS1 as a model probiotic strain.
机译:提出了一种评估单个颗粒内微生物生存力的实验方法,该方法包括单滴干燥方法和随后的新颖的活死筛选方法。干燥方法涉及将沉积在平坦疏水表面上的单滴干燥至确定的干燥条件和载体配方。(半)干燥的颗粒进行了水合,荧光染色和活/死枚举,采用了荧光显微镜技术。新颖的筛选方法提供了准确的存活百分比,与常规的平板枚举相符,并在单个样品中进行了评估植物乳杆菌WCFS1作为模型益生菌菌株的微滴干燥实验。

著录项

  • 来源
  • 会议地点 Xiamen(CN)
  • 作者单位

    Food Process Engineering Group,Wageningen UR P.O.Box 8129,6700 EV Wageningen,The Netherlands;

    Laboratory of Microbiology,Wageningen UR Building 316,Dreijenplein 10,6703 HB Wageningen,The Netherlands;

    NIZO Food Research P.O.Box 20,6710 BA Ede,The Netherlands;

    Food Process Engineering Group,Wageningen UR P.O.Box 8129,6700 EV Wageningen,The Netherlands;

    Host-Microbe Interactomics Group,Wageningen UR P.O.Box 338,6700 AH Wageningen,The Netherlands;

    Food Process Engineering Group,Wageningen UR P.O.Box 8129,6700 EV Wageningen,The Netherlands;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 空气处理;
  • 关键词

    probiotics; inactivation; drying; enumeration; single droplet;

    机译:益生菌;灭活;干燥;计数;单滴;

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