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SPOUTED BED DRYING AS A METHOD FOR ENZYME IMMOBILIZATION

机译:喷出床干燥作为酶固定化的一种方法

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摘要

Immobilization is usually a requirement to the use of enzymes as an industrial biocatalyst.In this work,endophytic fungus Cercospora kikuchii lipase was immobilized by covalent binding on agricultural by-products and microcrystalline cellulose.The enzyme-support system was dried in spouted bed.Microcrystalline cellulose with 1.5% of glutaraldehyde showed the best support,maintaining 179.1% of activity after drying,followed by the rice straw (173.9%),corn stover (169.8%),sugarcane bagasse (157.3%),green coconut fiber (102.3%) and corn cob (99.8%).The use of these immobilized derivatives obtained after spouted bed drying may help to increase the productivity while minimizing product recovery cost.
机译:固定化酶通常是使用酶作为工业生物催化剂所必需的。在这项工作中,内生真菌Cercospora kikuchii脂肪酶通过与农业副产物和微晶纤维素的共价结合而固定化。酶支持体系在喷射床中干燥。含戊二醛1.5%的纤维素显示出最佳的支持,干燥后保持179.1%的活性,其次是稻草(173.9%),玉米秸秆(169.8%),蔗糖蔗渣(157.3%),绿色椰子纤维(102.3%)。喷床干燥后获得的这些固定化衍生物的使用可能有助于提高生产率,同时最大程度地降低产品回收成本。

著录项

  • 来源
  • 会议地点 Xiamen(CN)
  • 作者单位

    University of S(a)o Paulo,Faculty of Pharmaceutical Sciences of Ribeir(a)o Preto Av.do Café s,Ribeir(a)o Preto,P.O.B.14040901,Brazil;

    University of S(a)o Paulo,Faculty of Pharmaceutical Sciences of Ribeir(a)o Preto Av.do Café s,Ribeir(a)o Preto,P.O.B.14040901,Brazil;

    University of S(a)o Paulo,Faculty of Pharmaceutical Sciences of Ribeir(a)o Preto Av.do Café s,Ribeir(a)o Preto,P.O.B.14040901,Brazil;

    University of S(a)o Paulo,Faculty of Pharmaceutical Sciences of Ribeir(a)o Preto Av.do Café s,Ribeir(a)o Preto,P.O.B.14040901,Brazil;

    University of S(a)o Paulo,Faculty of Pharmaceutical Sciences of Ribeir(a)o Preto Av.do Café s,Ribeir(a)o Preto,P.O.B.14040901,Brazil;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 空气处理;
  • 关键词

    spouted bed; enzyme immobilization; lipase; Cercospora kikuchii; agricultural by-products;

    机译:喷床;固定化酶;脂肪酶;菊苣菜;农业副产品;

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