Purification and Characterization of Cellulase from Trichoderma reesei

机译：里氏木霉纤维素酶的纯化和鉴定

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The influences of chromatography parameters on the resolution were studied in detail to determine the optimal conditions for purification. The purified endoglucanase was obtained by gel filtration of Superdex 75 prep grade with an activity recovery of 92.8% and the purification factor 4.2. The optimum catalysis temperature and pH for the enzyme were 55℃ and 4.5-5.0, respectively. The cellulase was stable at pH ranging from 4.0 to 6.0 and temperature below 60℃.

机译：详细研究了色谱参数对分离度的影响，以确定纯化的最佳条件。通过Superdex 75制备级的凝胶过滤获得纯化的内切葡聚糖酶，其活性回收率为92.8％，纯化因子为4.2。该酶的最佳催化温度为55℃，最适pH为4.5-5.0。纤维素酶在pH 4.0至6.0和温度低于60℃时稳定。

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来源
《The Third China-Korea conference on separation science and technology》|2001年|p.601-604|共4页
会议地点 Hangzhou(CN);Hangzhou(CN)
作者
YAO Shanjing; YU Lihua; GUAN Yixin;
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作者单位

Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027, China;

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会议组织
原文格式 PDF
正文语种 eng
中图分类化学工业;
关键词
cellulase; endoglucanase; purification; characterization; gel chromatography;

机译：纤维素酶;内切葡聚糖酶;纯化;表征;凝胶色谱;

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专利

1. Characterization of Crude Cellulase from Trichoderma reesei and Purification of Cellulase [J] . YAO Shanjing, GUAN Yixin, YU Lihua Chinese Journal of Chemical Engineering . 2002,第6期

机译：里氏木霉粗纤维素酶的表征和纤维素酶的纯化
2. Characterizationof Crude Cellulase from Trichoderma reesei and Purification of Cellulase [J] . 姚善泾, 关怡新, 等中国化学工程学报：英文版 . 2002,第006期

机译：里氏木霉粗纤维素酶的表征和纤维素酶的纯化
3. Expression and characterization of a novel metagenome-derived cellulase Exo2b and its application to improve cellulase activity in Trichoderma reesei [J] . Geng A., Zou G., Yan X., Applied Microbiology and Biotechnology . 2013,第4期

机译：一种新型的基因组纤维素酶Exo2b的表达，表征及其在里氏木霉中改善纤维素酶活性的应用
4. Purification and Characterization of Cellulase from Trichoderma reesei [C] . YAO Shanjing, YU Lihua, GUAN Yixin China-Korea conference on separation science and technology . 2001

机译：来自Trichoderma Reesei的纤维素酶的纯化和表征
5. Cellulase production by Trichoderma reesei Rut C30. [D] . Lo, Chi-Ming. 2008

机译：里氏木霉Rut C30生产纤维素酶。
6. Characterization of a copper responsive promoter and its mediated overexpression of the xylanase regulator 1 results in an induction-independent production of cellulases in Trichoderma reesei [O] . Xinxing Lv, Fanglin Zheng, Chunyan Li, 2015

机译：铜响应启动子的表征及其介导的木聚糖酶调节剂1的过表达导致里氏木霉中纤维素酶的诱导非依赖性产生
7. Cellulóz gének kifejeződése Trichoderma reesei-ben: a laktóz általi indukció mechanizmusa = Cellulase gene expression in Trichoderma reesei: mechanism of lactose induction [O] . Fekete Erzsébet 2008

机译：里氏木霉纤维素酶基因表达：乳糖诱导的机理=里氏木霉纤维素酶基因表达：乳糖诱导的机理
8. Papain digestion of crude Trichoderma reesei cellulase: Purification and properties of cellobiohydrolase I and II core proteins [R] . Woodward, J, Brown, J P, Evans, B R, 1992

机译：木瓜蛋白酶消化粗里氏木霉纤维素酶：纤维二糖水解酶I和II核心蛋白的纯化和性质

Purification and Characterization of Cellulase from Trichoderma reesei

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