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Tomographic microscopy of 3D phase objects with spatially incoherent illumination

机译:空间不相干照明的3D相位物体的断层显微镜

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Abstract: New method of a tomographic microscopy with spatially incoherent illumination is proposed. Earlier, in our works for reconstruction of a refractive index with spatial distribution of 3D phase samples, a method of tomographic microscopy with the coherent light was used. Main disadvantage of this scheme is use of a coherent light, which brings to appearance the phase noise in projection data. Spatially incoherent tomographic microscope is based on the Linnik phase-shifting interference microscope reflected type. Using interferometry with large source of the monochromatic light allows greatly improve a quality of phase projections by averaging of interference patterns. For tomographic mode of Linnik microscope working and obtaining the oblique illumination of sample method for displacing a light source is used. Observation angle range for 100 $MUL oil-immersion objective, N.A. equals 1.25, is 90 degrees. This method allows realizing a two-dimensional scanning trajectory for sample observation. Particularities of tomography for the phase objects, placed near plane mirror, are considered. So, a viewing angle range can be reduced to 90 degree, and sample is complement by its mirror reflection. The iterative algorithms for limited-angle tomographic reconstruction were used. Experimental results on three-dimensional reconstruction of the single human blood cell (erythrocyte) are presented. !31
机译:摘要:提出了一种在空间上不相干照明的断层显微镜的新方法。早先,在我们用于重建3D相样本空间分布的折射率的工作中,使用了一种采用相干光的断层显微镜的方法。该方案的主要缺点是使用了相干光,这使投影数据中的相位噪声显现出来。空间不相干断层扫描显微镜基于Linnik相移干涉显微镜反射型。将干涉仪与大量单色光一起使用,可以通过平均干涉图案来大大提高相位投影的质量。对于Linnik显微镜的X射线断层摄影模式,采用获取样品的倾斜照明的方法来替换光源。 100 $ MUL油浸物镜的观察角范围N.A.等于1.25,为90度。该方法允许实现用于样品观察的二维扫描轨迹。考虑放置在平面镜附近的相位对象的层析成像的特殊性。因此,视角范围可以减小到90度,并且样品通过其镜面反射得到补充。使用了用于有限角度层析成像重建的迭代算法。提出了单个人血细胞(红细胞)的三维重建的实验结果。 !31

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