• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VI >Managing multiple image stacks from confocal laser scanning microscopy
【24h】

Managing multiple image stacks from confocal laser scanning microscopy

机译：通过共聚焦激光扫描显微镜管理多个图像堆栈

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Abstract: A major goal in neuroanatomy is to obtain precise information about the functional organization of neuronal assemblies and their interconnections. Therefore, the analysis of histological sections frequently requires high resolution images in combination with an overview about the structure. To overcome this conflict we have previously introduced a software for the automatic acquisition of multiple image stacks (3D-MISA) in confocal laser scanning microscopy. Here, we describe a Windows NT based software for fast and easy navigation through the multiple images stacks (MIS-browser), the visualization of individual channels and layers and the selection of user defined subregions. In addition, the MIS browser provides useful tools for the visualization and evaluation of the datavolume, as for instance brightness and contrast corrections of individual layers and channels. Moreover, it includes a maximum intensity projection, panning and zoom in/out functions within selected channels or focal planes (x/y) and tracking along the z-axis. The import module accepts any tiff-format and reconstructs the original image arrangement after the user has defined the sequence of images in x/y and z and the number of channels. The implemented export module allows storage of user defined subregions (new single image stacks) for further 3D-reconstruction and evaluation. !3
机译：摘要：神经解剖学的主要目标是获得有关神经元组件及其相互连接的功能组织的精确信息。因此，组织切片的分析通常需要高分辨率图像以及有关结构的概述。为了克服这种冲突，我们先前已经引入了一种用于在共聚焦激光扫描显微镜中自动采集多个图像堆栈（3D-MISA）的软件。在这里，我们描述了一种基于Windows NT的软件，可快速轻松地浏览多个图像堆栈（MIS浏览器），单个通道和图层的可视化以及用户定义的子区域的选择。此外，MIS浏览器还提供了有用的工具来可视化和评估数据量，例如各个层和通道的亮度和对比度校正。此外，它还包括选定通道或焦平面（x / y）内最大强度的投影，平移和放大/缩小功能以及沿z轴的跟踪。在用户定义了x / y和z中的图像序列以及通道数之后，导入模块可以接受任何tiff格式并重建原始图像排列。实施的导出模块允许存储用户定义的子区域（新的单个图像堆栈），以进行进一步的3D重建和评估。 ！3

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号