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首页> 外文会议>Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VI >Spatial and temporal single-cell volume estimation by a fluorescence imaging technique with application to astrocytes in primary culture
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Spatial and temporal single-cell volume estimation by a fluorescence imaging technique with application to astrocytes in primary culture

机译：通过荧光成像技术估算时空单细胞体积并将其应用于原代培养的星形胶质细胞

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Abstract: Cell volume changes are often associated with importantphysiological and pathological processes in the cell.These changes may be the means by which the cellinteracts with its surrounding. Astroglial cells changetheir volume and shape under several circumstances thataffect the central nervous system. Following anincidence of brain damage, such as a stroke or atraumatic brain injury, one of the first events seen isswelling of the astroglial cells. In order to studythis and other similar phenomena, it is desirable todevelop technical instrumentation and analysis methodscapable of detecting and characterizing dynamic cellshape changes in a quantitative and robust way. We havedeveloped a technique to monitor and to quantify thespatial and temporal volume changes in a single cell inprimary culture. The technique is based on two- andthree-dimensional fluorescence imaging. The temporalinformation is obtained from a sequence of microscopeimages, which are analyzed in real time. The spatialdata is collected in a sequence of images from themicroscope, which is automatically focused up and downthrough the specimen. The analysis of spatial data isperformed off-line and consists of photobleachingcompensation, focus restoration, filtering,segmentation and spatial volume estimation. !42
机译：摘要：细胞体积的变化通常与细胞中重要的生理和病理过程有关，这些变化可能是细胞与其周围环境相互作用的方式。在影响中枢神经系统的几种情况下，星形胶质细胞会改变其体积和形状。在发生中风或无创伤性脑损伤等脑损伤后，星形胶质细胞肿胀是最早见到的事件之一。为了研究这种现象和其他类似现象，希望开发出能够以定量和鲁棒的方式检测和表征动态细胞形状变化的技术仪器和分析方法。我们已经开发出一种技术来监视和量化单个细胞原代培养物中的时空体积变化。该技术基于二维和三维荧光成像。时间信息是从一系列显微镜图像中获得的，并对其进行实时分析。从显微镜收集一系列图像中的空间数据，然后自动将其聚焦在样品上。空间数据分析是离线进行的，包括光漂白补偿，焦点恢复，滤波，分段和空间体积估计。 ！42

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