Spatial and temporal single-cell volume estimation by a fluorescenceimaging technique with application to astrocytes in primary culture,

机译：通过荧光成像技术估算时空单细胞体积，并将其应用于原代培养的星形胶质细胞，

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Abstract: Cell volume changes are often associated with important physiological and pathological processes in the cell. These changes may be the means by which the cell interacts with its surrounding. Astroglial cells change their volume and shape under several circumstances that affect the central nervous system. Following an incidence of brain damage, such as a stroke or a traumatic brain injury, one of the first events seen is swelling of the astroglial cells. In order to study this and other similar phenomena, it is desirable to develop technical instrumentation and analysis methods capable of detecting and characterizing dynamic cell shape changes in a quantitative and robust way. We have developed a technique to monitor and to quantify the spatial and temporal volume changes in a single cell in primary culture. The technique is based on two- and three-dimensional fluorescence imaging. The temporal information is obtained from a sequence of microscope images, which are analyzed in real time. The spatial data is collected in a sequence of images from the microscope, which is automatically focused up and down through the specimen. The analysis of spatial data is performed off-line and consists of photobleaching compensation, focus restoration, filtering, segmentation and spatial volume estimation. !42

机译：摘要：细胞体积的变化通常与细胞中重要的生理和病理过程有关。这些变化可能是细胞与其周围环境相互作用的方式。在几种影响中枢神经系统的情况下，星形胶质细胞会改变其体积和形状。在发生中风或外伤性脑损伤等脑损伤后，首先看到的事件之一是星形胶质细胞肿胀。为了研究这种现象和其他类似现象，希望开发出能够以定量和鲁棒的方式检测和表征动态细胞形状变化的技术仪器和分析方法。我们已经开发了一种技术，可以监视和量化原代培养中单个细胞的时空体积变化。该技术基于二维和三维荧光成像。时间信息是从一系列显微镜图像中获得的，并对其进行实时分析。空间数据是从显微镜中按一系列图像收集的，显微镜会自动在样品上上下对其进行聚焦。空间数据的分析是离线进行的，包括光漂白补偿，焦点恢复，滤波，分割和空间体积估计。！42

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《Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VI》|1999年|p.169-178|
会议地点 San Jose CA(US)
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Siamak Khatibi; Chalmers Univ. of Technology; Gothenburg; Sweden; Louise Allansson; Goeteborg Univ.; Gothenburg; Sweden; Tomas Gustavsson; Chalmers Univ. of Technology; Gothenburg; Sweden; Fredrik Blomstrand; Goeteborg Univ.; Gothenburg; Sweden; Elisabeth;
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1. Single-cell volume estimation by three-dimensional wide-field microscopy applied to astroglial primary cultures. [J] . Allansson L, Khatibi S, Gustavsson T, Journal of Neuroscience Methods . 1999,第1期

机译：通过三维宽视野显微镜对星形胶质原代培养物进行单细胞体积估计。
2. Inhibition of regulatory volume decrease in swollen rat primary astrocyte cultures by methylmercury is due to increased amiloride-sensitive Na+ uptake. [J] . Vitarella D, Kimelberg HK, Aschner M Brain research . 1996,第1a2期

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3. Metallothionein induction protects swollen rat primary astrocyte cultures from methylmercury-induced inhibition of regulatory volume decrease. [J] . Vitarella D, Conklin DR, Kimelberg HK, Brain research . 1996,第2期

机译：金属硫蛋白的诱导保护肿胀的大鼠原代星形胶质细胞培养物免受甲基汞诱导的调节体积减少的抑制。
4. Spatial and temporal single-cell volume estimation by a fluorescence imaging technique with application to astrocytes in primary culture [C] . Author(s): Siamak Khatibi Chalmers Univ. of Technology Gothenburg Sweden, Louise Allansson Goeteborg Univ. Gothenburg Sweden, Tomas Gustavsson Chalmers Univ. of Technology Gothenburg Sweden, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VI . 1999

机译：通过荧光成像技术估算时空单细胞体积并将其应用于原代培养的星形胶质细胞
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Spatial and temporal single-cell volume estimation by a fluorescenceimaging technique with application to astrocytes in primary culture,

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