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首页> 外文会议>Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIII; Progress in Biomedical Optics and Imaging; vol.7 no.13 >Effects of a pupil filter on Stimulated Emission Depletion Microscopy
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Effects of a pupil filter on Stimulated Emission Depletion Microscopy

机译：瞳孔滤光片对受激发射耗竭显微镜的影响

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摘要

Recently, stimulated emission depletion microscopy has achieved high resolution in fluorescent imaging. In this paper, we present effects of a pupil filter on the performances of stimulated emission depletion microscopy. In stimulated emission depletion microscopy, a saturated zero-centered spot is usually used to achieve a high lateral resolution. Using a half-coated phase plate, a zero-centered spot was made with a narrow and steep gap at the center. Numerical and experimental results show that by simply inserting a central obstacle as a pupil filter, it is possible to reduce the central gap of the zero-centered spot. However in order to compensate inevitable loss of light, which is blocked by the obstacle, increased laser power is required.
机译：最近，受激发射耗尽显微镜在荧光成像中已达到高分辨率。在本文中，我们介绍了瞳孔滤光片对受激发射耗竭显微镜性能的影响。在受激发射耗尽显微镜中，通常使用饱和零中心点来实现高横向分辨率。使用半涂层相板，制得零中心点，中心处有一个狭窄而陡峭的间隙。数值和实验结果表明，仅通过插入中心障碍物作为光瞳过滤器，就可以减小零中心点的中心间隙。然而，为了补偿被障碍物阻挡的不可避免的光损失，需要增加激光功率。

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