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首页> 外文会议>μTAS 2002 Symposium on Micro Total Analysis Systems 2002 Vol.2 Nov 3-7, 2002 Nara, Japan >SYNCHRONIZATION OF BACTERIAL CULTURES IN A MICROSYSTEM
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SYNCHRONIZATION OF BACTERIAL CULTURES IN A MICROSYSTEM

机译：微系统中细菌文化的同步

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摘要

A method is presented to size-fractionate and hence synchronize a mixed population of the rod-shaped bacterium Methylobacterium extorquens AM1, which exhibit a direct relationship between length (size) and age. Preliminary studies have shown that M. extorquens AM1 cells readily respond to negative and positive dielectrophoresis (DEP) and align parallel to the field vectors in a uniform AC field. When these bacteria were placed in a liquid cell medium flowing through a microfluidic channel, they were observed to interact with physical obstacles present inside the channel. A novel MEMS-based device was designed, fabricated, and tested to combine the effects of electrokinetic alignment and steric hindrance for the size-fractionation (synchronization by age) of these bacteria. The method demonstrated in this device is suitable for integration into lab-on-a-chip instrumentation for the analysis of selected metabolites and intermediates in non-spherical non-aggregating microorganisms.
机译：提出了一种对大小进行分级的方法，从而同步了棒状细菌勒索杆菌AM1的混合种群，该种群表现出长度（大小）与年龄之间的直接关系。初步研究表明，M。extorquens AM1细胞很容易对负和正介电电泳（DEP）作出反应，并在统一的交流场中平行于场矢量排列。将这些细菌置于流过微流体通道的液体细胞培养基中时，观察到它们与通道内存在的物理障碍物相互作用。设计，制造和测试了一种新颖的基于MEMS的设备，以结合电动对准和空间位阻对这些细菌的大小分级（按年龄同步）的影响。该设备演示的方法适合集成到芯片实验室仪器中，用于分析非球形非聚集微生物中的选定代谢物和中间体。

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