HIGH-THROUGHPUT SCREENING OF ANTICANCER DRUGS USING MICROARRAY BASED CELL CHIP

机译：使用基于微阵列的细胞芯片对抗癌药进行高通量筛选

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This paper describes fabrication of a microarray based cell chip and its suitability for cultivating human cancer cells (HeLa) in aqueous medium. Apart from this, the fabricated cell chip was also applied in high-throughput screening of anticancer activity against HeLa 229 cells of known standard carotenoids and carotenoid chemicals isolated from bacterial cultures. The anticancer or cytotoxic activity of these targeted chemicals was monitored by a fluorogenic substrate-calcein. The imaging was carried out using a chip scanner and the anticancer activity was expressed in terms of cell death. These fabricated microarray based cell chips play a profound role in high-throughput screening of potential anticancer drugs.

机译：本文介绍了基于微阵列的细胞芯片的制造及其在水性培养基中培养人癌细胞（HeLa）的适用性。除此之外，所制造的细胞芯片还用于高通量筛选针对已知标准类胡萝卜素和从细菌培养物中分离出的类胡萝卜素化学物质的HeLa 229细胞的抗癌活性。这些目标化学物质的抗癌或细胞毒性活性通过荧光底物钙蛋白酶进行监测。使用芯片扫描仪进行成像，并以细胞死亡表示抗癌活性。这些基于微阵列的加工细胞芯片在高通量筛选潜在抗癌药物中发挥着重要作用。

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来源
《μTAS 2002 Symposium on Micro Total Analysis Systems 2002 Vol.2 Nov 3-7, 2002 Nara, Japan》|2002年|p.862-864|共3页
会议地点 Nara(JP);Nara(JP)
作者
Sathuluri Ramachandra Rao; Yoshinori Akagi; Yasutaka Morita; Eiichi Tamiya;
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作者单位

School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1, Asahidai, Tatsunokuchi, Ishikawa 923-1292, Japan;

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会议组织
原文格式 PDF
正文语种 eng
中图分类 Q8;
关键词
high-throughput screening; microarray; cell chip; anticancer drugs;

机译：高通量筛选;芯片;细胞芯片;抗癌药物;
入库时间 2022-08-26 14:10:04

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HIGH-THROUGHPUT SCREENING OF ANTICANCER DRUGS USING MICROARRAY BASED CELL CHIP

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