声明
Table of Contents
Dedication
List of acronyms and abbreviation
Abstract
摘要
INTRODUCTION
CHAPTER 1
1．Literature reviews
1．1．Rice
1．2．Rice diseases
1．3 Fungi
1．4 Rhizoctonia spp
1．5 Mycoviruses
1．6．Control of plant fungal disease
1．7．Objective of this study
CHAPTER 2
2．Materials and Methods
2．1．Fungus isolate and culture condition
2．2．dsRNA extraction
2．3 Biological features of strains carrying dsRNA factors
2．4．Total RNA extraction
2．5．Particles purification and cDNA clone
2．6．Full-length cDNA sequence analysis
2．7．Northern blot analysis
2．8．Virus-like particles(VLPs)purification
CHAPTER 3
3．Results
3．1．Rhizoctonia strains isolated from rice and wheat diseases show diversity in colony morphology
3．2．Internal Transcribed Spacer Identification of the Isolates
3．3．dsRNA extraction from 40-1
3．4．Partial cDNA sequence of dsRNA segments(strain40-1)
3．5．Northern blotting analysis
3．6．Predicted secondary structure of mitovirus 3’-and 5’-UTR
3．7．VLP purification from strain 40-1
3．8．Molecular characterization of a novel partitivirus
3．9．Biological features of strain 40-1
CHAPTER 4
4．Discussion
References
Acknowledgment
