INHERITANCE,FINE MAPPING AND CANDIDATE GENE ANALYSIS OF RESISTANCE TO SOYBEAN MOSAIC VIRUS STRAINS SC5 AND SC20 INSOYBEAN(Glycine max(L.)Merr.)

声明

TABLE OF COINTENTS

LIST OF TABLES

LIST OF FIGURES

ABSTRACT

LIST OF ABBREVIATIONS

CHAPTER 1 LITERATURE REVIEW

1．1．1．Soybean

1．1．2．Soybean importance

1．2．SOYBEAN PRODUCTION AND ITS CONSTRAINTS

1．2．3．Viruses threaten the soybean production

1．3．SOYBEAN MOSAIC VIRUS，A MAJOR THREAT

1．3．1 Historical background and spread of SMV

1．3．2 SMV Taxonomy and strains

1．3．3．Molecular biology of SMV

1．3．4．Insect vectors of SMV

1．3．5．Source of inoculum and disease development

1．3．6．Synergism with other viruses

1．3．7．Impact of SMV infection on Soybean

1．4．DISEASE MANAGEMENT

1．5．HOST PLANT RESISTANCE

1．5．1．The nature of host plant resistance

1．5．2．Introgession of host plant resistance

1．6．GENOMICS-ASSISTED BREEDING(GAB)

1．7．MOLECULAR MARKERS AND GENETIC LINKAGE MAPS IN SOYBEAN

1．7．1．Molecular markers resources for soybean

1．7．2．Genetic linkage maps in soybean

1．8．GENE MAPPING

1．8．1．Bulk segregant analysis followed by traditional mapping

1．8．2．Genotyping-by-sequencing(GBS)

1．9．SOURCES，GENETICS AND GENES FOR RESISTANCE TO SMV

1．9．1．Sources and genetics of resistance to SMV

1．9．2．Genes for resistance to SMV

1．10．RESEARCH BACKGROUND AND OBJECTIVES

CHAPTER 2 INHERITANCE，FINE-MAPPING AND CANDIDATE GENE ANALYSIS OF THE GENE RESISTANT TO SMV STRAIN SC5

2．1．INTRODUCTION

2．2．MATERIALS AND METHODS

2．2．3．Inoculation and resistance evaluation

2．2．4．statistical analysis

2．2．5．Construction of genetic bin map and gene mapping

2．2．6．Association analysis and candidate gene selection

2．2．7．Quantitative Real Time-PCR(QRT-PCR)for expression profiling

2．3．RESULTS

2．3．3．Identification of genomic region associated with resistance gene to SC5

2．3．4．Candidate genes identification

2．3．5．Expression profiling of candidate genes

2．3．6．Summary of results

CHAPTER 3 INHERITANCE，FINE-MAPPING AND CANDIDATE GENE ANALYSIS OF THE GENE RESISTANT TO SMV STRAIN SC20

3．1．INTRODUCTION

3．2．MATERIALS AND METHODS

3．2．1．Plant materialsand source of strain

3．2．2．Inoculation and phenotypic assesment

3．2．3．DNA extractions，choice of SSR markeand mapping techniques

3．2．4．Data and linkage analysis

3．2．6．Fine-mapping and molecular markers development

3．2．7．Candidate gene prediction

3．2．9．Amplification and sequencing of resistance gene candidates

3．2．10．Amino acid prediction and sequence alignment

3．3．RESULTS

3．3．1．Inheritance analysis of resistance to SC20 in Qihuang．No．1

3．3．2．Identification of genomic region associated with resistance gene to SC20

3．3．3．Delimitation of a candidate genomic region

3．3．4．Candidate gene details

3．3．5．Expression profiling of candidate genes

3．3．6．Sequence analysis of resistance gene candidates

3．3．7．Summary of results

CHAPTER 4 DISCUSSION

4．2．ADVANTAGES OF THE GBS BASED BIN MAP STRATEGY

4．4．SMV RESISTANCE GENES IN CHROMOSOME 2(LG D1b)

4．5．MOST POSSIBLE CANDIDATE GENE TO SC5 RESISTANCE

4．6．STRATEGY FOR MAPPING OF SC20 RESISTANCE GENE

4．8．COMPARISON OF SC20 RESISTANCE GENE WITH OTHER SMV RESISTANCE GENES IN CHROMOSME 13(LG-F)

4．9．MOST POSSIBLECANDIDATE GENES TO SC20 RESISTANCE

4．10．CONCLUSION

CHAPTER 5 MAJOR INNOVALTION POINTS

5．3． CANDIDATE GENOMIC REGION AND MOST POSSIBLE CANDIDATE GENES TO SC20 RESISTANCE

REFERENCES

Appendices

ACKNOWLEDGEMENT

DEDICATION

BIOGRAPHICAL DATA