声明
CONTENTS
ABSTRACT
摘要
INTRODUCTION
1.0 Background
1.1 Justification of Research Work
1.2 Purpose of study
MATERIALS AND METHODS
2.0 Materials
2.1 Methods
2.2 Immunohistochemistry
2.3 Western Blot
2.4 Plasmid Construction and Extraction
2.5 Cell Culture and Transfection
2.6 Reporter Gene Analysis for Signaling Pathways
2.7 Cell Proliferation and Cytotoxicity Assay
2.8 Colony Formation Assay
2.9 Cell Cycle Analysis
2.10 DAPI(4,6-diamino-2-phenyl indole)staining
2.11 Migration and Invasion Assay
2.12 Stastistical Analysis
RESULTS
3.0 Immunohistochemistry(IHC)
3.1 Jakmip1 Protein Expression Level in Lung Tissues
3.2 Plasmid DNA Synthesis
3.3 Overexpression of Jakmip1
3.4 Cell Proliferation and Viability Assay
3.5 Colony Formation Assay
3.6 Jakmip1 Upregulation Activates Wnt/Beta Catenin Pathway
3.7 Beta-Catenin Expression In Transfected Cell Lines
3.8 Effect Jakmip1 Overexpression on Cyclin D1
3.9 PCNA Expression After Jakmip1 Overexpression
3.10 Cell Cycle Analysis
3.11 DAPI Staining ForApoptosis
3.12 Effect of Jakmip1 Overexpression on Bax Protein Expression
3.13 Effect of Jakmip1 on Bcl-2 Expression
3.14 Migration and Invasion Assay
DISCUSSION
4.0 Jakmip1 Expression in Lung Tumor Tissues
4.1 Jakmip1 Impact on Cell Proliferation
4.2 Jakmip1 Overexpression and Wnt Pathway Activity
4.3 Beta Catenin Expression
4.4 Influence of Higher Jakmip1 Expression on Cyclin D1
4.5 Proliferating Cell Nuclear Antigen(PCNA)Protein Expression
4.6 Cell Cycle Analysis
4.7 Effect of Jakmip1 Overexpression on Apoptosis
4.8 Bax and Bcl-2 Expression Level Upon Jakmip1 Upregulation
4.9 Jakmip1 Higher Expression and Cancer Cell Metastasis
CONCLUSION
REFERENCES
LITERATURE REVIEW
APPENDICES
DEDICATION
PERSONAL PUBLICATIONS
ACKNOWLEDGEMENTS
大连医科大学;