EVL-14/PDS-5和SCC-3是线虫有丝分裂和减数分裂必需的姐妹染色单体粘连蛋白

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ABstract

摘要

图表目录

CHAPTERⅠ

IntroductiontothemodelorganismCaenorhabditiselegans

Origins of the model

Powerful biology model

Genome

Genetics

Neurobiology

CHAPTERⅡ

CaenorhabditiseleganssisterchromatidcohesionproteinsEVL-14/PDS-5andSCC-3arerequiredforbothmeiosisandmitosis

Introduction

Results

Mutations in evl-14 and scc-3 disrupted the development ofthe vulva and gonad

evl-14andscc-3encodethelikelyhomologsoftheyeastPds5andScc respectively

Loss ofevl-14 and scc-3 gene functions disrupt sister chromatid cohesionduring meiosis

The localization of REC-8 to chromosomes is completely disruptedin scc-3 mnce

scc-3RNAiresultsinembryoniclethalityand evl-14/pds-5 RNAi causes a range of phenotypes

evl-14/pds-5 is expressed in embryo and postembryonic dividing cells

Discussion

Pvl Ste mutants primarily represent genes involved in cell divisionand cell cycle regulation

EVL-14/PDS-5 and SCC-3 function in both mitosis and meiosis

SCC-3 is essential for sister chromatid cohesion

EVL-14/PDS-5 is important for maintaining sister chromatid cohesionin late prophase

Model of cohesin

Materials and methodsStrains

C elegans Strains

Genetic mapping and molecular cloning

Double-stranded RNAi

Gonad preparation and immunostaining

DAPI staining and FISH

Microscopy

GFP reporter construction

ACKNOWLEDGMENTS

REFERENCES

PUBLICATIONS