声明
摘要
Abstract
Table of Contents
Chapter 1 Introduction
1．1 Current Status of Research and Application in Vascular Stents
1．1．1 Bare metal stents
1．1．2 Drug eluting stents
1．1．3 Biodegradable stents
1．1．4 EPC-capture stents
1．1．5 Time sequence functional stents
1．2 Surface Modification of Cardiovascular Materials and Implants
1．2．1 Blood-biomaterialinterfacial events and interaction mechanism
1．2．2 Surface modification for improving hemocompatibility
1．2．3 Biomimic endothelialization promoted surface modification
1．3 Research Progress of Plasma Polymerization in Biomedical Materials
1．3．1 A brief introduction of plasma and plasma polymerization
1．3．2 Altemative deposition of different functional groups
1．3．3 Plasma polymerization surface modification for biomedical materials
1．4 Significance and Objectives，Dissertation Layout and Technical route
1．4．1 Significance and objectives of research
1．4．2 Dissertation layout
1．4．3 Technical route
Chapter 2 Multifunctional Plasma Polymerized Film：Towards Better Anticorrosion Property，Enhanced Cellular Growth Ability，and Attenuated Inflammatory Responses
2．1 Abstract
2．2 Introduction
2．3 Experimental Section
2．3．1 Chemicals and reagents
2．3．2 Preparation of plasma polymerized films
2．3．3 Surface characterization
2．3．4 Quantification of amine groups by acid orange Ⅱ
2．3．5 Electrochemical corrosion tests
2．3．6 Protein adsorption via micro-BCA assays and QCM-D measurement
2．3．7 In vitro cellular responses
2．3．8 Anti-inflammatory effects
2．3．9 In vivo animal tests
2．3．10 Statistical analysis
2．4 Results
2．4．1 Surface characterization
2．4．2 Electrochemical corrosion measurements
2．4．3 Protein adsorption
2．4．4 Cell Morphology，adhesion and viability
2．4．5 Macrophage response and cytokine release
2．4．6 In vivo animal study
2．5 Discussion
2．6 Conclusion
Chapter 3 Improvement of Corrosion Resistance and Biocompatibility of BiodegradabIe Metallic Vascular Stent via Plasma Allylamine Polymerized Coating
3．1 Abstract
3．2 Introduction
3．3 Experimental Section
3．3．1 Chemicals and reagents
3．3．2 Preparation of plasma polymerized films
3．3．3 Surface characterization of PPAam coated MgZnMn and Fe samples
3．3．4 Electrochemical tests and analysis of corrosion products
3．3．5 ECs responses in direct culture with biodegradable coated metals
3．3．6 Morphology examination and balloon expansion tests
3．4 Results and Discussion
3．4．1 Physico-chemical properties of PPAam coated MgZnMn and Fe
3．4．2 Electrochemical corrosion behavior and analysis of corrosion products
3．4．3 Adhesion and proliferation of ECs
3．4．5 Morphology examination and balloon expansion tests
3．5 Conclusions
Chapter 4 Immobilization of DNA Aptamers via Plasma Polymerized Allylamine Film to Construct an Endothelial Progenitor Cell-Capture Surface
4．1 Abstract
4．2 Introduction
4．3 Experimental and Section
4．3．1 Chemicals and reagents
4．3．2 Preparation of plasma polymerized films
4．3．3 Immobilization of the EPC-aptamer
4．3．4 Surface characterization
4．3．5 QCM-D real time monitoring of aptamer immobilization
4．3．6 Platelet adhesion and morphology
4．3．7 Cellisolation．culture and identification
4．3．8 Cell capture and proliferation behavior
4．3．9 Competitive adhesion of EPC／EC and EPC／SMC via co-culture
4．3．10 Statistical analysis
4．4 Results and Discussion
4．4．1 Surface morphology roughness and wettability
4．4．2 Chemical surface compositions and quantitative characteristics
4．4．3 Platelet adhesion and morphology
4．4．4 Selective cell attachment and proliferation
4．5 Conclusion
Chapter 5 In-situ Release of Nitric Oxide Combined Heparinized Plasma Polymerized Allylamine Films for Constructing Multifunctional Hemocompatible Surface and Accelerating Endothelialization
5．1 Abstract
5．2 Introduction
5．3 Experimental Section
5．3．1 Chemicals and reagents
5．3．2 Preparation of plasma polymerized films
5．3．3 Sequential immobilization of heparin and SeCA
5．3．4 Quantification of amine groups
5．3．5 Analysis of chemical structure and compositions
5．3．6 QCM-D real time monitoring of sequential immobilization process
5．3．7 Catalytic release of NO behavior
5．3．8 cGMP analysis．anti-Xa assays and whole blood culture
5．3．9 ECs，SMCs and EPCs culture
5．3．10 Adhesion and proliferation of ECs，SMCs and EPCs
5．3．11 Effect of NO on mobilization of EPCs via transwell plate
5．3．12 Competitive adhesion of EC／SMC and EPC／SMC via co-culture
5．3．12 Statistical analysis
5．4 Results and Discussion
5．4．1 Sequential coimmobilization of heparin and SeCA on PPAam coating
5．4．2 Dynamic detection of NO release from PPAam-Hep-SeCA surface
5．4．3 cGMP analysis，anti-Xa assay and whole blood culture tests
5．4．4 Synergistic effects of heparin and NO on vascular cell growth behavior
5．5 Conclusion
Chapter 6 Summary and Future Research
6．1 Summary of Thesis
6．2 Limitation of Current Research
6．3 Recommendation for Future Research
Acknowlegements
References
Abbreviation List
Scientific achievements
