声明
摘要
ABSTRACT
TABLE OF CONTENT
LIST oF FIGURES
LIST OF TABLES
ABBREVIATION
CHAPTER ONE INTRODUCTION
1．1 The Cotton
1．1．1 Cotton morphology
1．1．2 Origin of cotton
1．1．3 Countries with Highest Production of Cotton
1．1．4 Agronomic properties for cotton production
1．1．5 Growth and development of a cotton plant
1．1．6 Uses of Cotton
1．2 Wild cotton species
1．2．1 Importance of wild cotton species in breeding
1．2．2 The use of wild cotton species：G．davidsonii and G．klotzschianum
1．3 Breeding and breeding strategies in cotton production
1．3．1 Development of parental lines
1．3．2 Genetic markers
1．4 Genetic Mapping
1．4．1 Significance of genetic mapping
1．4．2 Importance of SSR markers in screening and genotyping of population
1．4．3 Identification of polymorphism using SSR markers
1．4．4 Advantages of the use of EST-SSR markers
1．5 Distribution of genes on the D genome
1．6 Significance of gene discovery and mining in cotton genomes
1．7 Main objective of the research
1．7．1 Specific objectives
CHAPTER TWO MATERIALS AND METHODS
2．1 Plant materials
2．2 DNA extraction，quantification and qualification
2．3 Electrophoresis
2．4 Screening SSR markers for polymorphism
2．5 Genotyping using the SWU polymorphie primers
2．6 Software’s for construction of genetic map
2．7 Collinearity Analysis
2．8 Gene Mining，GO Functional Annotation，and Expression Analysis
CHAPTER THREE RESULTS
3．1 Marker information
3．2 General information about the genetic map
3．3 Segregation distortion
3．4 Collinearity analysis
3．5 Collinearity Analysis of the Genetic Map and the Physical Map of the (Dt) Sub-Genome
3．6 Gene Mining，GO Functional Annotation，and RNA sequence expression analysis
3．6．1 Gene mining
3．6．4 Gene Ontology (GO) analysis
3．6．5 RNA expression analysis
CHAPTER FOUR DISCUSSION
4．1 Parental materials
4．2 Polymorphism using EST-SSR markers
4．3 The genetic map constructed from wild species of the D genome
4．4 Segregation distortion in diploid cotton of D genome
4．5 Collinearity in D Genome cotton
4．6 Gene Mining，GO Annotation and Expression
4．6．1 Gene Mining
4．6．2 Physiological properties of mined genes
4．6．3 GO Annotations
CONCLUSION
REFERENCES
ACKNOWLEDGEMENT
CURRICULUM VITAE
中国农业科学院;
