声明
List of Abbreviations
Chapter 1General introduction
1.1 Background
1.2 Research status and progress of research in pollen of Chinese fir tree
1.3 Objectives
1.3.1 The overall objective
1.3.2 Specific objectives
1.4 Research questions
1.5 Innovation
Chapter 2Literature review
2.1 Chinese fir
2.1.1 Botanical description of Chinese fir tree
2.1.2 Flowering habit of Chinese fir
2.1.3 Distribution and habitat of Chinese fir
2.1.4 Uses of Chinese fir tree species
2.2 Pollen grains
2.2.1 Pollen morphology
2.2.2Aperture pattern in pollen grains
2.2.3 Exine ornamentation pattern in pollen grains
2.3 Pollen viability
2.3.1 In vitro pollen germination
2.3 .2 Pollen tube germination
2.3 .3 In vivo pollen germination
2.4 Effects of sucrose, boric acid and pH on pollen germination (PG)and pollen tube growth (PTG)
2.4.1 Effect of sucrose concentrations on PG and PTG
2.4.2 Effect of boric acid concentrations on PG and PTG
2.4.3 Effect of pH levels on PG and PTG
2.5 Metabolomics analysis
2.5.1 Metabolites profiling during pollen development
2.5.2 Secondary metabolites
2.6 Pollination
2.6.1 Controlled pollination (CP)
2.6.2 Cones and seeds characteristics
2.6.3 Seed germination and seedling establishment
Chapter 3Pollen morphology and structure analysis
3.1 Materials
3.1.1 The area of the study
3.1.2 Pollen samples
3.2 Methods
3.2.1 Preparation of the pollen for Scanning Electron Microscopy (SEM)
3.2.2. Pollen description and terminology
3.3 Results
3.3.1 General pollen morphological description
3.3. 2Apertures
3.3.3 Exine pattern
3.4 Discussion and conclusion
3.4.1 Pollen morphology
Chapter 4In vitro pollen germination and pollen tube growth
4.1 Materials
4.2 Methods
4.2.1 In vitro pollen germination
4.2.2 Pollen tube growth measurements (PTG)
4.2.3 Statistical analysis
4.3 Results
4.3.1 In vitro pollen germination on the basic media
4.3.2 Effects of sucrose, boric acid and pH on pollen germination (PG) and pollen tube growth (PTG)
4.4 Discussion and conclusion
4.4.1 Effects of sucrose concentration on PG and PTG
4.4.2 Effects of boric acid concentrations on PG and PTG
4.4.3 Effects of pH levels on PG and PTG
Chapter 5Metabolomics analysis during in vitro pollen germination
5.1 Materials
5.2 Methods
5.2.1 Determination of pollen developmental stages
5.2.2 Sample preparation
5.2.3 Liquid chromatography - mass spectrometry (LC / MS) Analysis
5.2.4 Data preprocessing and statistical analysis
5.2.5 Identification of differential metabolites
5.3 Results
5.3.1 Chromatograms
5.3.2 Multivariate statistical analysis
5.3.3 Screening of differential metabolites
5.3.4 Differences in the content of metabolites during pollen germination of Chinese fir tree
5.3.5 Enrichment analysis of differential metabolic pathways
5.3.6 The content changes of N-containing compounds in pollen during germination During the entire development of pollen grains, many secondary metabolites can
5.3.7 Analysis of endogenous hormones content changes in pollen during the germination
5.4 Discussion and conclusion
Chapter 6Controlled pollination
6.1 Materials
6.2 Methods
6.2.1 Controlled and open pollination experiments
6.2.2 Seed collection
6.2.3 Seed measurements
6.2.4 Seed germination
6.2.5 Data analysis
6.3 Results
6.3.1 Crossing of group (1) CL-13 (♀) × (♂)
6.3.2 Crossing of group (2) CL-4, 16 and 26 (♀) × (♂)
6.3.3 Crossing for group (3) CL- 4, 7, 13, 16 and 35 (♀) × (♂)
6.3.4 Group (4), open pollination (OP)
6.3.5 Seed germination, pollen germination and size for group (1)
6.3.6 Comparison of seed size for (OP) against controlled pollination (CP)
6.3.7 Comparison of seed characteristics for controlled pollination groups
6.3.8 Relationships between pollen, seed characteristics and seed germination
6.4 Discussion and conclusion
6.4.1 Effects of male and female parents on seeds characteristics
6.4.2 Relationships between pollen germination and pollen size and seeds characteristics
6.4.3 Relationships between seeds characteristics and seed germination
Chapter 7General conclusions and recommendations
7.1 Conclusions
7.2 Recommendations
参考文献
Published papers and other academic achievements during the program
致谢
Appendices
福建农林大学;
