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首页> 外文学位 >Biological functions of a blue-green alga, Nostoc commune var. sphaeroides Kutzing (N. commune).
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Biological functions of a blue-green alga, Nostoc commune var. sphaeroides Kutzing (N. commune).

机译：蓝绿藻Nostoc公社变种的生物功能。 sphaeroides Kutzing（公社）。

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摘要

Nostoc commune var. shproides Kutzing (N. commune), a blue-green alga, has been used as a medicinal food for centuries, but many of its biological functions have not been elucidated. The primary objective of this study was to determine the effect of N. commune on inflammation and cholesterol metabolism, several risk factors of atherosclerosis. Anti-inflammatory functions of N. commune were assessed using RAW 264.7 macrophages, a murine macrophage cell line. Incubation with N. commune lipid extract reduced pro-inflammatory markers such as tumor necrosis factor alpha (TNFalpha), cyclooxygenase 2 (COX-2), interleukin-1beta (IL-1beta) and inducible nitric oxide synthase (iNOS) in a nuclear factor-kappa B (NF-kappaB) dependent manner ( P 0.05). Contributing to its potential anti-antherogenic effect, N. commune reduced cholesterol biosynthesis in HepG2 cells, a human hepatoma cell line, by inhibiting the maturation of sterol regulatory element binding protein 2 (SREBP-2) and subsequently reducing the expression of its target genes, 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) and low-density lipoprotein receptor (LDLR). To further substantiate the role of N. commune as an athero-protective compound, C57BL/J6 mice were fed the AIN-93M diet supplemented with 0 or 5% N. commune. N. commune-fed mice exhibited reduced plasma total cholesterol concentrations by ∼20% (P = 0.003) as well as reduced cholesterol absorption in coordination with increased fecal neutral sterol output (P 0.05). The expression of cholesterol modulating genes, including SREBP-2 and HMGR, were significantly increased in mice fed N. commune ( P 0.030), indicating that N. commune intake affected hepatic cholesterol metabolism. In addition, the effects of blue-green alga intake on intestinal microflora were explored. Consumption of N. commune, Spriluna platensis and Aphanizomenon flos-aquae altered the gastrointestinal microbiota population in mice, with N. commune having the most pronounced effect on host microbiota. These data elucidate the molecular mechanisms by which N. commune may regulate both cholesterol metabolism and inflammation, providing evidence for N. commune to be used as a dietary supplement to lower the risk of coronary heart disease.
机译：诺斯托克公社shproides Kutzing（N. commune）是一种蓝绿色藻类，数百年来一直被用作药用食品，但其许多生物学功能尚未阐明。这项研究的主要目的是确定公猪对炎症和胆固醇代谢（动脉粥样硬化的几个危险因素）的影响。使用RAW 264.7巨噬细胞（一种鼠巨噬细胞系）评估了公猪猪笼草的抗炎功能。与公共猪笼草脂质提取物一起孵育可减少促炎性标志物，例如肿瘤坏死因子α（TNFalpha），环氧合酶2（COX-2），白介素1β（IL-1beta）和核因子中的诱导型一氧化氮合酶（iNOS）。 -kappa B（NF-kappaB）依赖方式（P <0.05）。通过抑制固醇调节元件结合蛋白2（SREBP-2）的成熟并随后减少其靶基因的表达，N。commune发挥了潜在的抗产瘤作用，从而降低了人类肝癌细胞系HepG2细胞中的胆固醇生物合成。 ，3-羟基-3-甲基戊二酰辅酶A还原酶（HMGR）和低密度脂蛋白受体（LDLR）。为了进一步证实公社猪作为动脉粥样硬化保护化合物的作用，向C57BL / J6小鼠喂食了补充有0或5％的公社猪的AIN-93M日粮。由公猪喂养的小鼠的血浆总胆固醇浓度降低了约20％（P = 0.003），胆固醇的吸收与粪便中性固醇输出的增加相协调（P <0.05）。饲喂猪笼草的小鼠中胆固醇调节基因（包括SREBP-2和HMGR）的表达显着增加（P <0.030），表明猪笼草的摄入影响了肝胆固醇的代谢。此外，还探讨了摄入蓝绿藻对肠道菌群的影响。食用N.公社，Spriluna platensis和Aphanizomenon flos-aquae会改变小鼠胃肠道菌群的数量，其中N.公社对宿主菌群的影响最为明显。这些数据阐明了N.公社可以调节胆固醇代谢和炎症的分子机制，为N.公社用作饮食补充品以降低冠心病风险提供了证据。

著录项

  • 作者

    Rasmussen, Heather E.;

  • 作者单位

    The University of Nebraska - Lincoln.;

  • 授予单位 The University of Nebraska - Lincoln.;
  • 学科 Biology Microbiology.;Health Sciences Nutrition.
  • 学位 Ph.D.
  • 年度 2008
  • 页码 177 p.
  • 总页数 177
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 微生物学;预防医学、卫生学;
  • 关键词

  • 入库时间 2022-08-17 11:39:05

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