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首页> 外文学位 >Development and application of a real-time polymerase chain reaction assay for the myxozoan parasite Henneguya ictaluri.
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Development and application of a real-time polymerase chain reaction assay for the myxozoan parasite Henneguya ictaluri.

机译：粘虫寄生虫Henneguya ictaluri的实时聚合酶链反应测定方法的开发和应用。

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摘要

Proliferative gill disease (PGD) caused by the myxozoan parasite Henneguya ictaluri is one of the most devastating parasitic infections in channel catfish aquaculture. Currently, there is no effective treatment for H. ictaluri and the unpredictable outbreaks can result in 100% mortality. Management strategies have been developed to prevent losses in newly stocked fingerlings by evaluating the PGD status of a pond prior to stocking, which is difficult since resident fish may not show clinical signs even when actinospore levels are lethal to naive fish. Current diagnostic methods are limited to the identification of an active infection and methods of predicting potential outbreaks have several limitations. The PGD status of a pond to be stocked can be determined using sentinel fish exposures which are labor intensive and require a source of parasite free fish. These limitations necessitated the development of more rapid and efficient means of determining actinospore concentrations to determine the risk of losing fish prior to stocking. The development of a quantitative real-time polymerase chain reaction (QPCR) assay provided a more rapid, sensitive and quantitative method of diagnosing active infections and also provides a means to predict potential PGD outbreaks and determine the PGD status of a pond prior to stocking.Another approach in the control of this parasite is the identification of a less susceptible culturable species or to identify traits that could be targeted in a selective breeding program. Challenge studies have shown that the closely related blue catfish (Ictalurus furcatus) does not exhibit as severe an inflammatory response to H. ictaluri and mortalities are significantly lower than in channel catfish. Comparisons of PGD severity and H. ictaluri infection in channel catfish, blue catfish and channel x blue catfish backcross hybrids by gross examination, histopathology and the newly developed H. ictaluri real-time PCR (QPCR) assay supported previous research suggesting the life cycle of the parasite can not be completed as efficiently through the blue catfish host.This dissertation describes the development and validation of a QPCR assay to detect H. ictaluri in both fish tissues and environmental samples and the application of this assay in both research and production settings.
机译：由粘虫寄生虫Henneguya ictaluri引起的增生性disease病（PGD）是channel鱼养殖中最具破坏性的寄生虫感染之一。目前，尚无有效的解决方法用于肉嗜血杆菌，而且无法预测的暴发可导致100％的死亡率。已经开发出管理策略，通过在放养前评估池塘的PGD状态来防止新放养的鱼种损失，这是困难的，因为即使活肌动孢子水平即使对幼稚鱼也具有致命性，常驻鱼类也可能没有临床迹象。当前的诊断方法仅限于活动性感染的鉴定，而预测潜在爆发的方法也有一些局限性。放养池塘的PGD状态可以通过劳动密集且需要无寄生鱼来源的前哨鱼暴露来确定。这些局限性使得必须开发出更快速有效的方法来确定放线孢子的浓度，以确定放养前失去鱼类的风险。实时定量聚合酶链反应（QPCR）测定法的发展为诊断活动性感染提供了一种更加快速，灵敏和定量的方法，还提供了一种预测潜在PGD爆发并确定放养前池塘PGD状态的方法。控制这种寄生虫的另一种方法是鉴定不太易感的可培养物种或鉴定在选择性育种计划中可能针对的性状。挑战性研究表明，密切相关的蓝cat鱼（Ictalurus furcatus）并未表现出严重的对鱼嗜血杆菌的炎症反应，死亡率显着低于槽channel鱼。通过粗略检查，组织病理学和新近开发的H. ictaluri实时荧光定量PCR（QPCR）分析比较了channel鱼，蓝cat鱼和x蓝cat鱼回交杂种中PGD的严重性和H. ictaluri感染，这支持了先前的研究，提示了其生命周期。通过蓝describes鱼宿主不能完全有效地完成该寄生虫的研究。本文描述了一种QPCR检测方法的开发和验证，该检测方法可检测鱼组织和环境样品中的鱼，并在研究和生产环境中应用。

著录项

  • 作者

    Griffin, Matthew John.;

  • 作者单位

    Mississippi State University.;

  • 授予单位 Mississippi State University.;
  • 学科 Biology Veterinary Science.Agriculture Fisheries and Aquaculture.
  • 学位 Ph.D.
  • 年度 2008
  • 页码 145 p.
  • 总页数 145
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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