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首页> 外文学位 >Green tea and grape seed extracts to control Listeria monocytogenes, Escherichia coli O157:H7 and Salmonella Typhimurium in whey protein edible film and mechanism of action of the extracts in inhibiting Listeria monocytogenes and Escherichia coli O157:H7.
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Green tea and grape seed extracts to control Listeria monocytogenes, Escherichia coli O157:H7 and Salmonella Typhimurium in whey protein edible film and mechanism of action of the extracts in inhibiting Listeria monocytogenes and Escherichia coli O157:H7.

机译：绿茶和葡萄籽提取物可控制乳清蛋白可食性膜中的单核细胞增生李斯特菌，大肠杆菌O157：H7和鼠伤寒沙门氏菌，以及提取物抑制单核细胞增生李斯特菌和大肠杆菌O157：H7的作用机理。

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摘要

Foodborne diseases caused by Listeria monocytogenes, E. coli O157:H7 and Salmonella Typhimurium have been associated with the consumption of a range of food products including ready-to-eat poultry and meat products. There is an increasing interest in natural therapies and consumer demand for effective, safe, natural products. Hence, it is important to investigate antimicrobial activities of plant extracts as potential sources of novel antimicrobials. Combinations of natural antimicrobials can serve as potential intervention strategies for controlling foodborne pathogens. Also, understanding mode of action of grape seed extract (GSE) and green tea extract (GTE) is important if they are to find commercial application as natural preservatives. Therefore, the objectives of this study were to evaluate the inhibitory effect of GSE and GTE combined with nisin (N) and malic acid (MA) in whey protein edible films against the three major pathogens in the turkey frankfurter system and to investigate the mode of action of GTE and GSE in inhibiting the pathogens. GSE and GTE had synergistic effects when combined with N and MA and lowered the Listeria monocytogenes population by 4.6 log cycles after 28 days in turkey frankfurters stored at 4 °C. No such synergistic effects between the extracts and N and MA were observed in Salmonella Typhimurium and E. coli O157:H7. MA alone lowered the Salmonella Typhimurium population by 3.3 log cycles compared to the control, while the combination of N, MA, GSE and EDTA lowered the counts by 2.0 log cycles only compared to the control. The E. coli O157:H7 population was lowered by 4.6 log cycles compared to the control, while combination of N, MA, GSE and EDTA lowered the counts by 3.4 log cycles. GSE and GTE had bactericidal effects on Listeria monocytogenes and E. coli O157:H7 at concentrations of more than 4 and 6 mg/ml, respectively. At bactericidal concentrations GSE and GTE resulted in a high percentage (90%) of cells with propidium iodide uptake in Listeria monocytogenes and E. coli O157:H7, respectively. Cell killing was accompanied by depletion in the ATP pools of the cells due to cellular cytoplasmic leakage, indicating that the bactericidal activity of GSE and GTE results from damage to the cytoplasmic membrane. Both GSE and GTE inhibited the membrane bound ATPase activity significantly at concentrations of more than 2 and 6 mg/ml in Listeria monocytogenes and E. coli O157:H7, respectively.
机译：由单核细胞增生性李斯特菌，大肠杆菌O157：H7和鼠伤寒沙门氏菌引起的食源性疾病与食用一系列食品有关，包括即食家禽和肉类产品。天然疗法和消费者对有效，安全，天然产品的需求日益增长。因此，重要的是研究植物提取物的抗菌活性，作为新型抗菌剂的潜在来源。天然抗菌素的组合可作为控制食源性病原体的潜在干预策略。另外，如果要获得天然防腐剂的商业用途，了解葡萄籽提取物（GSE）和绿茶提取物（GTE）的作用方式也很重要。因此，本研究的目的是评估GSE和GTE结合乳链菌肽（N）和苹果酸（MA）在乳清蛋白可食用膜中对火鸡法兰克福体系中三种主要病原体的抑制作用，并研究其模式。 GTE和GSE在抑制病原体中的作用。当将GSE和GTE与N和MA结合使用时，具有协同作用，并且在4°C的火鸡法兰克福香肠中放置28天后，单核细胞增生李斯特菌的数量降低了4.6 log个周期。在鼠伤寒沙门氏菌和大肠杆菌O157：H7中未观察到提取物与N和MA之间的这种协同作用。与对照相比，单独使用MA可使鼠伤寒沙门氏菌种群减少3.3个对数周期，而与对照相比，N，MA，GSE和EDTA的结合仅使计数减少2.0个对数周期。与对照组相比，大肠杆菌O157：H7种群减少了4.6个对数周期，而N，MA，GSE和EDTA的组合使计数减少了3.4个对数周期。 GSE和GTE分别以超过4和6 mg / ml的浓度对单核细胞增生李斯特菌和大肠杆菌O157：H7具有杀菌作用。在杀菌浓度下，GSE和GTE分别导致单核细胞增生性李斯特菌和O157：H7大肠杆菌中高比例（90％）的碘化丙啶摄取细胞。由于细胞质泄漏，细胞的杀伤伴随着细胞ATP池的耗竭，这表明GSE和GTE的杀菌活性是由对细胞质膜的破坏导致的。在单核细胞增生李斯特菌和大肠杆菌O157：H7中，当浓度分别超过2和6 mg / ml时，GSE和GTE均显着抑制膜结合的ATPase活性。

著录项

  • 作者

    Chitturi, Vidya.;

  • 作者单位

    University of Arkansas.;

  • 授予单位 University of Arkansas.;
  • 学科 Agriculture Food Science and Technology.
  • 学位 Ph.D.
  • 年度 2008
  • 页码 191 p.
  • 总页数 191
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 农产品收获、加工及贮藏;
  • 关键词

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