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Characterization of cryocapacitation in boar sperm and how seminal plasma proteins affect this process.

机译：公猪精液中冷冻致死的特征以及精浆蛋白如何影响这一过程。

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摘要

After nearly 30 years of commercially available frozen-thawed (FT) boar sperm, it has not received widespread acceptance for commercial breeding in the swine industry. Currently, less than 1% of all artificial insemination in the swine industry is performed using FT semen. This is a result of the suboptimal fertility rates obtained when FT semen is used. Boar semen has proven difficult to cryopreserve. The sperm cells are extremely vulnerable to cold shock and undergo a capacitation-like process during cryopreservation, termed cryocapacitation, leading to decreased fertility. In this dissertation, we compared capacitation and cryocapacitation and examine the effects of seminal plasma (SP) on these processes, identified protein components of SP that are responsible for the suppression of capacitation and cryocapacitation, and characterized the Crisp family of genes as well as the function of these proteins to regulate capacitation and cryocapacitation. We demonstrated that SP has a regulatory effect on both capacitation and cryocapacitation and provided evidence for the proteins in SP being responsible for this result. We have also shown the similarities between the two processes. The major results of this dissertation are twofold. First, cryocapacitation results in a similar endpoint as capacitation as defined by the competency to undergo an acrosome reaction. We hypothesize that the pathways that each process takes are different. Second, specific proteins in SP can alter and/or regulate these pathways. We have shown that members of the spermadhesin protein family and not the Crisp family of proteins are involved in this inhibition in the boar.
机译：经过近30年的商业化冻融（FT）公猪精子，它在猪业中尚未得到商业繁殖的广泛认可。目前，在猪业中，只有不到1％的人工授精是使用FT精液进行的。这是使用FT精液时获得的次佳生育率的结果。公猪精液已被证明很难冷冻保存。精子极易受到冷冲击，并在冷冻保存过程中经历了类似capacitation的过程，称为冷冻失活，导致生育能力下降。在本文中，我们比较了获能和低温致死，并研究了精浆（SP）对这些过程的影响，鉴定了可抑制获能和致冷致死的SP蛋白成分，并表征了Crisp家族基因以及这些蛋白质在调节获能和冻融中的功能。我们证明了SP对获能和冻融均具有调节作用，并提供了SP中蛋白质负责此结果的证据。我们还显示了两个过程之间的相似之处。本文的主要研究结果是双重的。首先，冷冻吞噬导致的终止作用与获能所经历的顶体反应能力定义的终止作用相似。我们假设每个过程采取的途径是不同的。其次，SP中的特定蛋白质可以改变和/或调节这些途径。我们已经表明，精子粘附蛋白家族的成员而不是Crisp家族的成员参与了公猪的这种抑制作用。

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  • 作者

    Vadnais, Melissa L.;

  • 作者单位

    University of Minnesota.;

  • 授予单位 University of Minnesota.;
  • 学科 Agriculture Animal Culture and Nutrition.; Biology Veterinary Science.
  • 学位 Ph.D.
  • 年度 2007
  • 页码 113 p.
  • 总页数 113
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 饲料;动物学;
  • 关键词

  • 入库时间 2022-08-17 11:39:20

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