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EARLI1 proteins localize to the cell wall and protect cells from freezing damage.

机译：EARLI1蛋白位于细胞壁，并保护细胞免受冰冻损害。

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摘要

Plants possess a large family of proteins sharing a conserved eight-cysteine-domain motif (8CM). Members of the 8CM family play important roles in plant growth, reproduction, and responses to both abiotic and biotic stresses. However, the biochemical functions of these proteins are largely unknown. Therefore, as part of my thesis I decided to biochemically analyze the 8CM-containing, hybrid proline-rich protein (HyPRP) EARLI1. EARLI1 is a cold responsive Arabidopsis gene that encodes a protein with a two-domain architecture: a proline-rich domain (PRD) at the N-terminus, and an 8CM domain at the C-terminus. I found that knocking down EARLI1 increased freezing-induced cellular damage. EARLI1-GFP fusion protein and immunocytochemical studies showed that the protein may be localized to the plant cell wall. Biochemical analyses showed that the PRD is hydrophilic, and that the 8CM domain is either hydrophobic or prone to aggregate in solution. Both domains have cysteine residues and produce higher-ordered complexes via disulfide bond formation. Higher ordered protein complexes recognized by anti-EARLI1 antiserum were also observed predominantly, but not exclusively, in cold acclimated plants. I propose that EARLI1 has a bimodular architecture in which the PRD interacts with the cell wall, and the 8CM domain with the plasma membrane. Stress induced bimodular proteins such as EARLI1 may play a role in protecting both the cell wall and the cell membrane when challenged by certain types of environmental stresses, including cold and possibly pathogen attack.
机译：植物拥有共享保守的八个半胱氨酸结构域基序（8CM）的一大类蛋白质。 8CM家族的成员在植物生长，繁殖以及对非生物和生物胁迫的响应中起着重要作用。但是，这些蛋白质的生化功能很大程度上未知。因此，作为本论文的一部分，我决定对包含8CM的富含脯氨酸的杂合蛋白（HyPRP）EARLI1进行生化分析。 EARLI1是一种冷应答拟南芥基因，其编码具有两个域结构的蛋白质：N端富含脯氨酸的域（PRD），C端具有8CM的域。我发现敲除EARLI1会增加冰冻诱导的细胞损伤。 EARLI1-GFP融合蛋白和免疫细胞化学研究表明，该蛋白可能位于植物细胞壁上。生化分析表明，PRD是亲水的，并且8CM结构域是疏水的或易于在溶液中聚集。两个结构域均具有半胱氨酸残基，并通过二硫键形成产生更高阶的复合物。还主要在冷驯化植物中观察到了但不是唯一地观察到抗EARLI1抗血清识别的高级蛋白质复合物。我建议EARLI1具有双模块架构，其中PRD与细胞壁相互作用，而8CM结构域与质膜相互作用。当受到某些类型的环境压力（包括寒冷和可能的病原体侵袭）挑战时，压力诱导的双模块蛋白（例如EARLI1）可能在保护细胞壁和细胞膜方面发挥作用。

著录项

  • 作者

    Zhang, Yi.;

  • 作者单位

    Marquette University.;

  • 授予单位 Marquette University.;
  • 学科 Biology Plant Physiology.
  • 学位 Ph.D.
  • 年度 2007
  • 页码 176 p.
  • 总页数 176
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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