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The effect of water stress on the expression of proteins in hard red spring wheat Triticum aestivum cv. Butte 86.

机译：水分胁迫对硬红春小麦普通小麦蛋白质表达的影响。比尤特86。

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摘要

Scope and Method of Study. Environmental changes including water stress affect the wheat quality and yield. This study attempts to explore the effect of water stress on the storage and specific organelle proteins' expression in hard red spring wheat Triticum aestivum cv Butte 86 during development. Wheat plants were grown in a greenhouse under optimum and 30% water-stressed conditions. Both plant treatments received adequate nitrogen fertilization and spikes were harvested at 14, 34 days after anthesis (DAA) as early and late stage of endosperm development respectively. The mature wheat grains were harvested at 60 DAA. Gliadin, LMW-GS, and HMW-GS of mature wheat were differentially extracted with solvents. Proteins characterizations were obtained by their hydrophobic properties via reverse phase-high performance liquid chromatography (RP-HPLC), mass to charge ratio using capillary zone electrophoresis (CZE), molecular weight via sodium dodecyl sulfate-polyacrelamide gel electrophoresis (SDS-PAGE), and isoelectric point and molecular weight using two-dimensional (2-D) gel electrophoresis. While the endoplasmic reticulum (ER) and golgi complex (GC) from 14 and 34 DAA were fractionated using sucrose gradient and analyzed by 2-D electrophoresis. Proteins were analyzed by peptide mass fingerprinting and identified with protein database.;Findings and Conclusions. Based on equal volume of extraction, higher relative amount of protein from RP-HPLC area of gliadins and LMW-GS of stressed compared to optimal fractions, while lower relative amount of protein concentration was observed in the stressed fractions of HMW-GS compared to optimal fractions, with the caveat that systematic errors in actual protein amount may occur. Water stress produced change in the expression of HMW-GS 5+10 which are encoded by the gene Glu-D1 by upregulating the Glu-D1x and downregulating the Glu-D1y. During early and late stages of development several starch synthesis proteins were identified accompanied with upregulation of the expression of stress and defense proteins. Dehydrin, Hsp26, Glutathione-S-Transferase, and receptor-like kinase among stress response proteins were good candidates to have an inhibition effect on starch synthesis enzymes that need to be further investigated.
机译：研究范围和方法。包括水分胁迫在内的环境变化都会影响小麦的品质和单产。本研究试图探索水分胁迫对硬红春小麦普通小麦Butte 86发育过程中贮藏和特定细胞器蛋白质表达的影响。小麦植株在温室中最佳水分胁迫条件下生长。两种植物处理均接受了足够的氮肥，并且在开花后（DAA）的第14、34天分别作为胚乳发育的早期和晚期收获了穗状花序。以60 DAA收获成熟小麦籽粒。用溶剂差分提取成熟小麦的麦醇溶蛋白，LMW-GS和HMW-GS。通过反相高效液相色谱（RP-HPLC）的疏水性，使用毛细管区带电泳（CZE）的质荷比，通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳的分子量（SDS-PAGE）获得蛋白质的表征使用二维（2-D）凝胶电泳分析等电点和分子量。使用蔗糖梯度分离14和34 DAA的内质网（ER）和高尔基复合体（GC），并通过2-D电泳进行分析。通过肽质谱指纹分析法对蛋白质进行分析，并通过蛋白质数据库进行鉴定。基于相等的提取量，与最佳馏分相比，麦醇溶蛋白的RP-HPLC区域中蛋白质的相对含量较高，而应激的LMW-GS的蛋白质相对含量较高，而与最佳成分相比，在HMW-GS的应激成分中观察到的蛋白质浓度较低注意，可能会发生蛋白质实际含量的系统错误。水分胁迫通过上调Glu-D1x和下调Glu-D1y而导致HMW-GS 5 + 10的表达发生变化，该变化由基因Glu-D1编码。在发育的早期和晚期，鉴定了几种淀粉合成蛋白，并伴随着应激和防御蛋白表达的上调。应激反应蛋白中的脱水蛋白，Hsp26，谷胱甘肽-S-转移酶和受体样激酶是对淀粉合成酶具有抑制作用的良好候选者，有待进一步研究。

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  • 作者

    Al Jorf, Fadi.;

  • 作者单位

    Oklahoma State University.;

  • 授予单位 Oklahoma State University.;
  • 学科 Agriculture Agronomy.;Agriculture Plant Culture.
  • 学位 Ph.D.
  • 年度 2008
  • 页码 117 p.
  • 总页数 117
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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