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首页> 外文学位 >Biosensing based on localized surface plasmon resonance of gold nanostructures fabricated by a novel nanosphere lithography technique .
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Biosensing based on localized surface plasmon resonance of gold nanostructures fabricated by a novel nanosphere lithography technique .

机译：基于新型纳米球面光刻技术的金纳米结构局部表面等离子体共振的生物传感。

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摘要

New gold nanostructures were prepared through a modified nanosphere lithography method. The structures, obtained by self-assembly of polystyrene microspheres and gold colloids in multilayers and subsequent removal of polystyrene, consist of nanohole/nanorings, distributed uniformly among gold nanoparticles on a silanized glass substrate. The size of the hole corresponds to the footprint of the polystyrene microspheres that were in contact with the glass substrate before dissolution. Sensing is based on the spectral measurement (in transmission mode) of the position of the Au localized surface plasmon resonance band in the ultraviolet-visible (UV-Vis) spectrum. Experiments were conducted with small molecules as well as with proteins (fibrinogen, Amyloid beta-derived diffusible ligands (ADDLs), and AT5G07010.1, a plant protein from Arabidopsis thaliana) incubated on a functionalized substrate and have shown a higher sensitivity for platforms with 100 and 200 nm holes, compared to those having larger (500 and 700 nm) holes; for example, by using a platform with 100 nm holes, a concentration as low as 4.3x10-7 M of the plant protein (AT5G07010.1) could be detected. The biorecognition of the plant protein and the corresponding antibody has been shown by the broadening of the localized surface plasmon resonance band and the presence of a new band around 600-620 nm. It is believed that this band originates in interparticle localized surface plasmon resonance coupling, when biorecognition forces the functionalized particles into close proximity.
机译：通过改进的纳米球光刻技术制备了新的金纳米结构。通过将聚苯乙烯微球和金胶体自组装成多层并随后去除聚苯乙烯而获得的结构由纳米孔/纳米结构组成，纳米孔/纳米结构均匀地分布在硅烷化玻璃基板上的金纳米颗粒之间。孔的尺寸对应于在溶解之前与玻璃基材接触的聚苯乙烯微球的足迹。感测基于在紫外可见（UV-Vis）光谱中Au定位表面等离子体激元共振带的位置的光谱测量（以透射模式）。实验是在功能化底物上孵育的小分子以及蛋白质（纤维蛋白原，淀粉样蛋白衍生的可扩散配体（ADDLs）和来自拟南芥的植物蛋白AT5G07010.1）进行的，并且显示了对具有与具有较大（500和700 nm）孔的孔相比，具有100和200 nm孔；例如，通过使用具有100 nm孔的平台，可以检测到低至4.3x10-7 M的植物蛋白（AT5G07010.1）浓度。植物蛋白和相应抗体的生物识别已通过局部表面等离振子共振带的加宽和600-620 nm附近新带的存在而得到证明。据信，当生物识别迫使功能化颗粒紧密接近时，该带起源于颗粒间局部表面等离子体共振耦合。

著录项

  • 作者

    Fida, Farah.;

  • 作者单位

    Concordia University (Canada).;

  • 授予单位 Concordia University (Canada).;
  • 学科 Engineering Computer.;Engineering Electronics and Electrical.
  • 学位 M.A.Sc.
  • 年度 2008
  • 页码 84 p.
  • 总页数 84
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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