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首页> 外文学位 >The effect of 5-aminolevulinic acid based photodynamic therapy and photochemical internalization of bleomycin on the F98 rat glioma cell line.
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The effect of 5-aminolevulinic acid based photodynamic therapy and photochemical internalization of bleomycin on the F98 rat glioma cell line.

机译：基于5-氨基乙酰丙酸的光动力疗法和博来霉素的光化学内在化对F98大鼠神经胶质瘤细胞系的影响。

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摘要

The F98 rat glioma cell line, a model for human malignant glioma, was used to investigate the efficacy of 5-aminolevulinic acid (ALA)-mediated photodynamic therapy (PDT) under varying light irradiation conditions. Cells in monolayer were pre-incubated in ALA for 4 hours prior to 635 nm light exposure. Using clonogenic survival assays, it was found that F98 glioma cells were sensitive to ALA-PDT: survival was inversely proportional to light fluence. As evidenced from the LD50 (4 J cm-2 at a fluence rate of 35 mW cm-2), ALA-PDT was found to be more effective in F98 cells compared to that found in another commonly used rat glioma cell line (BT4C). In contrast to findings in BT4C cells, F98 survival was independent of fluence rate.;ALA-PDT was tested for its ability to enhance the cytotoxicity of Bleomycin (BLM), a procedure termed photochemical internalization (PCI). F98 cells were incubated in ALA and BLM prior to illumination. In all cases, no difference in survival was observed between F98 cells subjected to ALA-PCI delivery of BLM and cells treated with ALA-PDT. These findings suggest that ALA is not an ideal compound for PCI applications.
机译：F98大鼠神经胶质瘤细胞系，人类恶性神经胶质瘤的模型，用于研究5-氨基乙酰丙酸（ALA）介导的光动力疗法（PDT）在不同光照条件下的功效。在635 nm曝光之前，将单层细胞在ALA中预孵育4小时。使用克隆发生存活测定法，发现F98胶质瘤细胞对ALA-PDT敏感：存活率与光通量成反比。从LD50（4 J cm-2，以35 mW cm-2的注量速度）可以证明，与其他常用的大鼠神经胶质瘤细胞系（BT4C）相比，ALA-PDT在F98细胞中更有效。 。与BT4C细胞中的发现相反，F98的存活率与注量率无关。ALA-PDT具有增强博莱霉素（BLM）细胞毒性的能力，该过程称为光化学内在化（PCI）。在照明前，将F98细胞在ALA和BLM中孵育。在所有情况下，在接受BLM的ALA-PCI递送的F98细胞和经ALA-PDT处理的细胞之间，均未观察到存活率差异。这些发现表明，ALA不是PCI应用的理想化合物。

著录项

  • 作者

    Kharkhuu, Khishigzaya.;

  • 作者单位

    University of Nevada, Las Vegas.;

  • 授予单位 University of Nevada, Las Vegas.;
  • 学科 Biophysics Medical.;Health Sciences Oncology.
  • 学位 M.S.
  • 年度 2008
  • 页码 40 p.
  • 总页数 40
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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