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首页> 外文会议>Conference on lasers in surgery: Advanced characterization, therapeutics, and systems >Repetitive 5-aminolevulinic acid mediated photodynamic therapy of rat glioma
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Repetitive 5-aminolevulinic acid mediated photodynamic therapy of rat glioma

机译：重复的5-氨基纤维酸介导的大鼠胶质瘤的光动力治疗

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The probability of achieving local control with current single-shot, intraoperative PDT treatments of intracerebral gliomas seems improbable due to the length of time required to deliver adequate light fluences to depths of 1 - 2 cm in the resection margin. The results of in vitro experiments indicated that PDT, repeated at weekly intervals, was substantially more effective at inhibiting glioma spheroid growth than single treatment regimes. This prompted the initiation of in vivo studies of repetitive PDT in a rat glioma model. BT_4C cell line tumors were established in the brains of inbred BD-IX rats. Three days following tumor induction, the animals were injected with 250mg/kg ALA i.p. and four hours later, after the introduction of an optical fiber, light treatment at various radiant energies was given over a 10- to 30-minute interval. Two additional treatments were given at weekly intervals. In vitro experiments verified that spheroids derived from the cell line were sensitive to ALA PDT. Microfluorometry of frozen tissue sections showed that PpIX is produced with a 10-20:1 tumor to normal tissue selectivity ratio four hours after 250 mg/kg ALA i.p. Toxic radiant energy levels for ALA PDT have been determined.
机译：由于在切除余量中为1 - 2厘米的深度为1 - 2厘米的深度所需的时间长度，脑脑脑膜脑膜脑膜炎术术中PDT治疗的局部对照，脑内脑膜炎术的术中PDT治疗的可能性似乎是不可能的。体外实验的结果表明，每周间隔重复的PDT在抑制胶质瘤球状生长时比单处理制度在抑制胶质瘤球状生长方面大致更有效。这促使对大鼠胶质瘤模型中重复PDT的体内研究开始。在近交BD-IX大鼠的大脑中建立了BT_4C细胞系肿瘤。肿瘤诱导后三天，将动物注射250mg / kg Ala I.P.四个小时后，在引入光纤之后，在10至30分钟的间隔内给出各种辐射能量的光处理。每周间隔给出另外两种治疗方法。体外实验证实，衍生自细胞系的球形对ALA PDT敏感。冷冻组织切片的微氟度测定表明，在250mg / kg Ala I.P中，用10-20：1肿瘤产生10-20：1肿瘤以正常组织选择性比。已经确定了ALA PDT的有毒辐射能量水平。

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