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首页> 外文学位 >Using biotechnology to improve the production of rosmarinic acid from rosemary plants.
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Using biotechnology to improve the production of rosmarinic acid from rosemary plants.

机译：使用生物技术改善迷迭香植物中迷迭香酸的生产。

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Rosemary (Rosmarinus officinalis) is a very important medical herb containing secondary products in its leaves. It belongs to the mint family (Lamiaceae), and is native to the Mediterranean region. Rosmarinic acid (RA) is a very strong antioxidant produced in rosemary leaves and used to protect plant tissues against free radicals and lipid peroxidation caused by stress. Also it is used in medicine, food preservatives and cosmetics. The objectives of my study was to (1) Measure the concentration of RA in leaves and callus of 5 different rosemary genotypes (Majorca, Rosmarinus officinalis, Pine Scented, Madeline Hill and Arp). (2) Increase the RA concentration in callus produced from leaf tissues by increasing the medium osmotic pressure using different sucrose concentrations or through enhancing the RA biosynthetic pathway (Increasing L-proline and L-phenylalanine in medium). (3) Measure the stress gene (PAL) expression in leaves of the five genotypes and its effect on RA production. It was found that the best genotypes for RA production from leaf tissues were Pine Scented and Majorca during the spring. While for RA extraction from callus it is preferred to use genotype Rosmarinus officinalis or Madeline Hill in the second subculture. Dark treatment and high sucrose concentration increased the RA content in callus tissues (0.0755 mg RA/gm fw callus) reaching level higher than the RA concentration inside leaf tissues. It is preferred to use sucrose concentration at 4% for Rosmarinus officinalis and 3% sucrose for Madeline Hill in the dark condition. Combination of L-proline (4 mM) and L-phenylalanine (0.8 gm/L) enhanced RA production in callus of Madeline Hill. It is preferred to extract the RA from callus tissues since in callus there is no tissue specific regulation which causes different PAL gene expression inside plant tissues due to exogenous and endogenous stimuli. Also it will enable the production of high amount of RA in relatively small areas; this approach potentially will save agricultural lands for the production of essential food crops.
机译：迷迭香（Rosmarinus officinalis）是非常重要的药用草药，其叶中含有次级产品。它属于薄荷家族（唇形科），原产于地中海地区。迷迭香酸（RA）是一种非常强的抗氧化剂，在迷迭香叶中产生，用于保护植物组织免受压力引起的自由基和脂质过氧化作用。也用于医药，食品防腐剂和化妆品。我的研究目的是（1）测量5种不同迷迭香基因型（马约卡，迷迭香，松香，马德琳·希尔和阿普）的叶片和愈伤组织中RA的浓度。 （2）通过使用不同的蔗糖浓度增加培养基的渗透压或通过增强RA的生物合成途径（增加培养基中的L-脯氨酸和L-苯丙氨酸）来增加叶片组织中愈伤组织的RA浓度。 （3）测量五种基因型的叶片中的胁迫基因（PAL）的表达及其对RA产生的影响。人们发现，春季从叶片组织中产生RA的最佳基因型是松香和马略卡岛。对于从愈伤组织中提取RA，优选在第二次传代培养中使用基因型迷迭香或玛德琳·希尔。深色处理和高蔗糖浓度会使愈伤组织中的RA含量增加（0.0755 mg RA / gm fw愈伤组织），其含量高于叶组织内部的RA浓度。在黑暗条件下，迷迭香的蔗糖浓度优选为4％，玛德琳山的蔗糖浓度为3％。 L-脯氨酸（4 mM）和L-苯丙氨酸（0.8 gm / L）的结合提高了Madeline Hill愈伤组织中RA的产生。优选从愈伤组织中提取RA，因为在愈伤组织中没有组织特异性调节，该调节由于外源和内源刺激而引起植物组织内不同的PAL基因表达。它还将使在相对较小的区域中产生大量的RA成为可能；这种方法有可能节省农业土地来生产必需的粮食作物。

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