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首页> 外文学位 >Multi-functional Centrifugal Microfluidic Discs for Rio-detection Applications.
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Multi-functional Centrifugal Microfluidic Discs for Rio-detection Applications.

机译：用于里约检测应用的多功能离心微流控盘。

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摘要

This work is motivated by the need of miniaturized and automated biomedical assay platforms in the field of point-of-care applications. The focus of microfluidic system for integrated biomedical assays has attracted more and more attention in recent years. Centrifugal microfluidics, or lab-on-a-disc (LOAD), is believed to be an excellent candidate to tackle complex biomedical analysis problems with a broad range of validated functional units such as valving, mixing and metering. However, it is still at early stage. While many individual biological analysis steps, functions, and systems have been demonstrated, there are relatively few examples of real LOAD. New developments targeting true sample-to-answer devices for biomedical assays will lead to wide-spread utilization of centrifugal microfluidic technologies as a valuable tool in the near future. The goal of this study is on the emphasis of new technical achievements towards establishing advanced centrifugal microfluidic disc-based analysis devices for various biomedical applications.;In this thesis, we have demonstrated a simple yet convenient disc to perform bead-based immunoassays. The disc embeds a series of passive valves and a beads damming structure onto a downscale network. Without beads pre-packaging for specific assays and expensive liquid dispensing system, a broad range of bead based immunoassays can be conducted flexibly on the disc in a rapid and low-cost manner. The integrated platform contains all the necessary functional elements thus allow one-step parallel assays through a frequency protocol. To prove our concept, quantitative analysis of ovalbumin of various concentrations was performed on the device. The integrated device only requires the addition of samples and a single run of device with a precise acceleration speed (3 rpm/s) to trigger a cascade of events that is controlled by the increasing speed, and it takes 5 min 33 s for the solutions to move entirely from the loading pads to the waste chamber followed by the detection. The beads (45 mum - 160 mum) with detection antibody are washed and then aggregated to the small targeted detection region with a small damming microchannel (40 mum) which allows free passage of small molecules while retaining the beads. Our work demonstrates the feasibility of using centrifugation as a possible automated immunoassay system in the future.
机译：这项工作是由即时医疗应用领域中小型化和自动化的生物医学检测平台的需求所推动的。近年来，用于集成生物医学测定的微流体系统的焦点已引起越来越多的关注。离心微流控或盘片实验室（LOAD）被认为是解决复杂生物医学分析问题的极佳选择，其具有多种经过验证的功能单元，例如阀门，混合和计量。但是，它仍处于早期阶段。尽管已演示了许多单独的生物学分析步骤，功能和系统，但实际LOAD的例子相对较少。针对用于生物医学测定的真正的从样品到答案的设备的新发展将导致离心微流控技术在不久的将来得到广泛使用。这项研究的目的是强调新的技术成就，以建立适用于各种生物医学应用的先进的基于离心微流控盘的分析设备。在本论文中，我们证明了一种简单而方便的盘可以进行基于珠子的免疫测定。圆盘将一系列的被动阀和一个隔珠结构嵌入到小型网络中。无需为特定的测定法预先包装珠子，也无需昂贵的液体分配系统，就可以以快速，低成本的方式灵活地在圆盘上进行多种基于珠子的免疫测定。集成平台包含所有必需的功能元件，因此允许通过频率协议进行一步并行分析。为了证明我们的概念，在设备上进行了各种浓度的卵白蛋白的定量分析。集成设备仅需要添加样本，即可运行一次具有精确加速速度（3 rpm / s）的设备，以触发一系列由速度增加控制的事件，而解决方案则需要5分33 s完全从装载垫移至废料仓，然后进行检测。洗涤带有检测抗体的小珠（45微米至160微米），然后通过一个小阻尼微通道（40微米）聚集到小的靶向检测区域，该微通道可允许小分子自由通过，同时保留微珠。我们的工作证明了将来使用离心作为可能的自动化免疫测定系统的可行性。

著录项

  • 作者

    Chen, Qiulan.;

  • 作者单位

    The Chinese University of Hong Kong (Hong Kong).;

  • 授予单位 The Chinese University of Hong Kong (Hong Kong).;
  • 学科 Engineering Biomedical.;Chemistry Biochemistry.;Engineering Electronics and Electrical.
  • 学位 Ph.D.
  • 年度 2010
  • 页码 176 p.
  • 总页数 176
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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