• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文学位 >Insights into the roles of metals in biology: Biochemical and structural characterization of two bacterial and one archaeal metallo-enzyme.
【24h】

Insights into the roles of metals in biology: Biochemical and structural characterization of two bacterial and one archaeal metallo-enzyme.

机译：洞察金属在生物学中的作用：两种细菌和一种古细菌金属酶的生化和结构表征。

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The focus of my research was functional characterization of three different protein systems by biochemical techniques and X-ray crystallography. The first project involved structural studies of the enzyme peptide deformylase (PDF) from Escherichia coli. What makes E. coli PDF different from other enzymes of the same class is its use of iron as opposed to zinc at its catalytic center. To elucidate the basis of this unique metal preference, we have solved high-resolution structures of PDF bound to three different metals---iron, cobalt, or zinc. Our structures reveal differences in how the product of the reaction---formate, binds to iron vs. zinc, and provide insight into PDF's mechanism of catalysis. These results have strong implications for gaining insights into the catalytic properties of PDF and subsequent development of antibiotics targeted against bacterial PDFs.; X-ray crystallography was also used to characterize proteins from a methane-generating microorganism called Methanosarcina barkeri. M. barkeri is an archaea that plays an essential role in the global carbon cycle by converting atmospheric carbon dioxide to methane. A previously unobserved amino acid, L-pyrrolysine was identified in the M. barkeri protein MtmB by our group and the laboratory of Dr. Joseph Krzycki (The Ohio State University, Microbiology). My focus in this collaborative project was to gather structural evidence for the proposed biological role of L-pyrrolysine in MtmB. As a part of this effort, we have determined the X-ray structure of the corrinoid protein MtmC that interacts with MtmB. Additionally, a model for the interaction between MtmB and MtmC was developed. This model is based on low-resolution X-ray data, homology modeling, and mass spectrometry.; The final system under investigation involves the study of putative protease networks in E. coli. Protein degradation is an important part of cellular metabolism in E. coli. Most of the initial steps of degradation are performed by ATP-dependent proteases: HflA, HflB (FtsH), Lon, and Clp (ClpXP, ClpAP, and ClpYQ or HslUV). The aim of this work is to explore a putative role of the peptidase oligopeptidase A as a downstream partner of these energy dependent proteases. Our results indicate that oligopeptidase A efficiently cleaves the peptides generated by the activity of three energy dependent proteases suggesting a potential role in multiple catabolic pathways.
机译：我的研究重点是通过生化技术和X射线晶体学对三种不同蛋白质系统的功能表征。第一个项目涉及大肠埃希氏菌酶肽去甲酰基化酶（PDF）的结构研究。使E. coli PDF与其他同类酶不同的原因是，在催化中心使用铁而不是锌。为了阐明这种独特的金属偏好的基础，我们解决了与三种不同金属（铁，钴或锌）绑定的PDF的高分辨率结构。我们的结构揭示了反应产物甲酸盐与铁与锌的结合方式的差异，并提供了PDF催化机理的见解。这些结果对于深入了解PDF的催化特性以及随后开发针对细菌PDF的抗生素具有重要意义。 X射线晶体学也被用来表征甲烷生成甲烷的微生物，称为甲烷甲烷菌。 barkeri菌是古细菌，它通过将大气中的二氧化碳转化为甲烷而在全球碳循环中发挥重要作用。我们的小组和Joseph Krzycki博士（俄亥俄州立大学微生物学）实验室在巴氏支原体蛋白MtmB中鉴定了以前未发现的氨基酸L-酪氨酸。在这个合作项目中，我的重点是收集L-吡咯赖氨酸在MtmB中拟议的生物学作用的结构性证据。作为这项工作的一部分，我们确定了与MtmB相互作用的类蜂蛋白MtmC的X射线结构。此外，开发了MtmB和MtmC之间相互作用的模型。该模型基于低分辨率X射线数据，同源性建模和质谱。研究中的最终系统涉及大肠杆菌中假定的蛋白酶网络的研究。蛋白质降解是大肠杆菌细胞代谢的重要组成部分。降解的大多数初始步骤均由ATP依赖性蛋白酶完成：Hf1A，Hf1B（FtsH），Lon和Clp（ClpXP，ClpAP和ClpYQ或HslUV）。这项工作的目的是探索肽酶寡肽酶A作为这些能量依赖性蛋白酶的下游伴侣的假定作用。我们的结果表明，寡肽酶A有效地裂解了由三种能量依赖性蛋白酶的活性产生的肽，表明在多种分解代谢途径中可能发挥作用。

著录项

  • 作者

    Jain, Rinku.;

  • 作者单位

    The Ohio State University.;

  • 授予单位 The Ohio State University.;
  • 学科 Biophysics General.
  • 学位 Ph.D.
  • 年度 2006
  • 页码 184 p.
  • 总页数 184
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 生物物理学;
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号