Examination of ADAR-RNA and ADAR-ADAR interactions.

机译：检查ADAR-RNA和ADAR-ADAR相互作用。

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RNA within a cell is subject to multiple processing events prior to undergoing translation, including RNA editing through the chemical modification of RNA bases. An example of this is adenosine deamination to produce inosine, a reaction carried out in mammalian cells by the adenosine deaminases that act on RNA (ADARs). The experiments presented in this thesis examine both ADAR - RNA and ADAR - ADAR interactions using in vitro and in vivo techniques. Through the use of footprinting we were able to identify regions on a model substrate which were protected upon binding of an ADAR2 homodimer. Utilizing fluorescence microscopy, we were able to determine that ADAR1 and ADAR2 form both homodimers and heterodimers within the nucleoli of HeLa cells. This thesis also reports our endeavors at characterizing novel extra-nucleolar regions to which ADAR1 was discovered to localize. The results within suggest methods by which adenosine deamination is regulated within the cell.

机译：细胞内的RNA在经历翻译之前会经历多种加工事件，包括通过化学修饰RNA碱基进行RNA编辑。这样的一个例子是产生肌苷的腺苷脱氨，肌苷是在哺乳动物细胞中通过作用于RNA（ADAR）的腺苷脱氨酶进行的反应。本文提出的实验使用体内和体外技术研究了ADAR-RNA和ADAR-ADAR的相互作用。通过使用足迹，我们能够确定模型基质上受ADAR2同型二聚体结合而受到保护的区域。利用荧光显微镜，我们能够确定ADAR1和ADAR2在HeLa细胞核仁中形成同二聚体和异二聚体。本论文还报告了我们在鉴定ADAR1被定位到的新型核仁外区域方面所做的努力。内结果提示了在细胞内调节腺苷脱氨的方法。

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作者
Lynch, Kaari Andrea.;
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University of Alberta (Canada).;

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授予单位 University of Alberta (Canada).;
学科 Chemistry Biochemistry.
学位 M.Sc.
年度 2006
页码 92 p.
总页数 92
原文格式 PDF
正文语种 eng
中图分类生物化学;
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Examination of ADAR-RNA and ADAR-ADAR interactions.

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