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Negative interference in systems of coupled kinesin: A study of self-assembling complexes with defined structure.

机译：耦合驱动蛋白系统的负干扰：具有确定结构的自组装复合物的研究。

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摘要

Intracellular transport is a crucial process that requires the work of motor proteins to distribute necessary cargos. Many times the motors must move over long distances and against high opposing forces than those generated by single motors. To accomplish this task motors appear to act in teams, as suggested by experiments that show enhanced force production and extended travel lengths. Many motors have been characterized individually, but experiments to study their collective mechanics rely on non-specific groupings where the copy number and geometric arrangement are not explicitly known. In order to resolve the true extent to which each motor contributes enhanced transport properties, a system must be developed that precisely controls the number of motors that are studied. Within this work, a convergent self-assembly approach is presented that allows structurally-defined complexes of kinesin-1 to be created. This approach also provides synthetic control over intermotor spacing and the elasticity of the mechanical motor linkages to rigorously characterize the effects of system structure on the interactions of exactly two motors. This synthetic coupled motor system was then used to examine the extent to which motor grouping enhances the transport properties of cargos. It was determined that the average velocity of coupled kinesin proteins was statistically indistinguishable from that of the single motor, while the average run lengths of the two-motor system were slightly longer (≈ 2X), but less than estimated for a system of non-interacting motors (≈ 4X). This study concludes that, under low loads, intermotor strain in coupled kinesin proteins increases the rate of motor detachment from the microtubule and decreases the rate at which additional motors rebind. The presence of negative interference in these complexes implies that groupings of kinesins preferentially travel in a single motor-attachment state, and that only a subset of cargo-bound motors are used during transport.
机译：细胞内运输是至关重要的过程，需要运动蛋白的工作来分配必要的货物。许多情况下，电动机必须比单电动机产生的力长距离运动并承受较大的反作用力。为了完成这项任务，电机似乎可以协同工作，如实验所示，该实验显示出增加的力量产生和更长的行进长度。许多电动机已经分别进行了表征，但是研究其集体力学的实验依赖于非特定分组，在这些分组中，拷贝数和几何排列方式并不清楚。为了解决每个电动机真正有助于提高运输性能的程度，必须开发一种系统来精确控制所研究电动机的数量。在这项工作中，提出了一种收敛的自组装方法，该方法允许创建结构定义的驱动蛋白1的复合物。这种方法还提供了对电动机之间的间距和机械电动机联动装置的弹性的综合控制，以严格表征系统结构对正好两个电动机的相互作用的影响。然后，使用该合成耦合电动机系统检查电动机分组在多大程度上增强了货物的运输性能。已确定，耦合的驱动蛋白的平均速度与单马达的平均速度在统计学上没有区别，而两马达系统的平均运行长度稍长（＆ap; 2倍），但小于非马达系统的估计速度。 -互动马达（＆ap; 4X）。这项研究得出的结论是，在低负载下，耦合的驱动蛋白中的马达间应变会增加马达与微管的分离速度，并降低其他马达重新结合的速度。这些复合物中存在负面干扰意味着驱动蛋白的组合优先以单个马达附着状态行进，并且在运输过程中仅使用一部分装满货物的马达。

著录项

  • 作者

    Rogers, Arthur Russell.;

  • 作者单位

    Rice University.;

  • 授予单位 Rice University.;
  • 学科 Chemistry Biochemistry.;Biophysics General.
  • 学位 Ph.D.
  • 年度 2010
  • 页码 90 p.
  • 总页数 90
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

  • 入库时间 2022-08-17 11:36:56

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